News Release
Two Journal Articles CiteCleanascite™ in Studies of Lipid Influences in Cell Response Assays
MONMOUTHJUNCTION, NJ,September9, 2021 -- Biotech Support Group reports on two articles describingthe simplicity and efficiency of their lipid depletion technology todetermine the influence of lipids in cellular response assays. Thefirst citation is:
Chen,Hsin-Yi, et al. "Selectiveabrogation of S6K2 maps lipid homeostasis as a survival vulnerabilityin MAPKi-resistant NRASmut melanoma." bioRxiv (2021).
Thearticle reports that silencing of the ribosomal protein S6 kinase 2(S6K2), while preserving the activity of S6K1, perturbs lipidmetabolism, enhances fatty acid unsaturation, triggers lipidperoxidation and induces cell death selectively in NRAS-mutantmelanoma cells that are resistant to MAPK inhibition. To helpidentify cell response factors, the article states “Lipid-depletedserum (FBS) was prepared by treating with Cleanascite reagent at avolume ratio of 1:4 per the manufacturer’s instructions.” Thestudy establishes S6K2 and its effector subnetwork as promisingtargets for NRAS-mutant melanoma that are resistant to global MAPKpathway inhibitors.
Thesecond citation is:
Pointner,Lisa, et al. "Birchpollen induces Toll-like receptor 4-dependent dendritic cellactivation favoring T cell responses." Frontiersin Allergy (2021):42.
Thisstudy aimed to examine (i) the importance of Toll-like receptor4 (TLR4) for dendritic cell (DC) activation by birch pollenextracts (BPE), (ii) the extent of the contribution of BPE-derivedlipopolysaccharide (LPS) and other potential TLR4 adjuvant(s) in BPE,and (iii) the relevance of the TLR4-dependent activation ofBPE-stimulated DCs in the initiation of an adaptive immune response.The article states “To remove the lipids in BPE, CleanasciteTM was used …according to manufacturer's recommendations … in aratio 1:1 (v/v). Importantly, “non-specifictreatment-associated and cytotoxic effects were ruled out …asneither the protein digestion nor the lipid extraction procedureaffected cell activation.” These findings suggest that TLR4 isa major pathway by which BPE triggers DC activation that is involvedin the initiation of adaptive immune responses.
“Asthese new citations attest, Cleanascite™ has become the go-toproduct for lipid removal in the most challenging applications.Whenever an investigation needs to consider whether lipids influencecell culture response, Cleanascite™ can be used. It is an aqueoussuspension product without harmful solvents, or any othernon-specificcytotoxic effects.This ultimately helps researchers decide whether or not lipids, orfactors interacting with lipids, impart characteristic changes incells.” states Swapan Roy, Ph.D., President and Founder of BiotechSupport Group
Clickto download whitepaper entitled “Cleanascite™- Lipid Removal and Cell Response Applications”
Clickto visit Cleanascite™product page and ordering information
AboutBiotech Support Group LLC
Convergingwith cultural and technological disruptions forthcoming inhealthcare, Biotech Support Group develops methods for cost effectiveand efficient sample prep essential for these expanding markets.Following a tiered business strategy, the company continues itsgrowth in the consumable research products area supporting therapidly expanding installation of LC-MS instrument and computationalinfrastructure. For this market, key products include: AlbuVoid™and AlbuSorb™ for albumin depletion, Cleanascite™ for lipidadsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal,and NuGel™ for functional proteomics. From these innovations, thecompany has acquired knowledgebase and biomarker intellectualproperty assets that support discoveries of protein markers fromblood, with special emphasis on early detection and personalizedmedical decisions for cancer patients. For more information, gotohttp://www.biotechsupportgroup.com.
ForBusiness Development,contact:MatthewKuruc 732-274-2866, [email protected]