NewsRelease
JournalArticle Cites AlbuSorb™ for Serum Exosome Proteomic Analysis
MONMOUTHJUNCTION, NJ,May14, 2018 -- Biotech Support Group reports on a recent journal articleusing AlbuSorb™to improve serum exosome purification for subsequent proteomicanalysis.
Thecitation is:
Chettimada,Sukrutha, et al. "Exosomemarkers associated with immune activation and oxidative stress in HIVpatients on antiretroviral therapy."ScientificReports8.1 (2018): 7227.
Theauthors investigated the role that exosomesplay in HIV pathogenesis, as little is known about exosome cargo inrelation to immune responses and oxidative stress. For this, plasmaexosomes in HIV patients and their relationship to immunological andoxidative stress markers were characterized by electron microscopy,nanoparticle tracking, immunoblotting, and LC-MS/MS proteomics. ForLC-MS/MS proteomics, thearticle states “Toobtain purified exosome fractions for proteomic analysis,… albuminwas depleted with 2 rounds of albumin depletion using AlbuSorb™ -Albumin Depletion Kit (Biotech Support Group)”. Using untargetedproteomic analysis, Exosomal Notch4 was increased in HIV-positivesubjects versus controls and correlated with immune activationmarkers. The results from this study suggest that exosomes inantiretroviral therapy-treated HIV patients carry proteins related toimmune activation and oxidative stress, have immunomodulatory effectson myeloid cells, and may have pro-inflammatory and redox effectsduring pathogenesis.
“Thisreference shows that while exosomes are large hydrophobic vesicles,separating them from surrounding protein content, can be difficult.Nevertheless, AlbuSorb™is demonstrated here to be useful in depleting albumin and enrichingexosomes. Thus LC-MS/MSproteomic analysisof the exosomes is not compromised by interference from serumalbumin.” states Swapan Roy, Ph.D., President and Founder ofBiotech Support Group.
Formore information visit: AlbuSorb™at
http://www.biotechsupportgroup.com/AlbuSorb-Albumin-Depletion-Kit-p/a185.htm
AboutBiotech Support Group LLC
Convergingwith cultural and technological disruptions forthcoming inhealthcare, Biotech Support Group develops methods for cost effectiveand efficient sample prep essential for these expanding markets.Following a tiered business strategy, the company continues itsgrowth in the consumable research products area supporting therapidly expanding installation of LC-MS instrument and computationalinfrastructure. For this market, key products include: AlbuVoid™and AlbuSorb™ for albumin depletion, Cleanascite™ for lipidadsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal,and NuGel™ for functional proteomics. From these innovations, thecompany has acquired knowledgebase and biomarker intellectualproperty assets that support discoveries of protein markers fromblood, with special emphasis on early detection and personalizedmedical decisions for cancer patients. For more information,go tohttp://www.biotechsupportgroup.com
Contact:
MatthewKuruc 732-274-2866