Research Article Cites Cleanascite™ for Mass Spectrometric Proteome Analysis of Human Bile Fluid

News Release

Research Article Cites Cleanascite™ for Mass Spectrometric Proteome Analysis of Human Bile Fluid

, January 4, 2017 -- Biotech Support Group reports on a recent research article describing the simplicity and efficiency of their proteomic sample preparation technology for clearing lipids from bile fluid.

The citation is:

Megger, Dominik A., et al. "One Sample, One Shot-Evaluation of sample preparation protocols for the mass spectrometric proteome analysis of human bile fluid without extensive fractionation." Journal of Proteomics (2016).

In brief, the article’s authors report methods to overcome the biological variability of analyzing a high number of bile samples. They advance that easy sample preparation protocols are demanded representing a compromise between proteome coverage and simplicity in this study. For this, they evaluated the performance of simple workflows allowing for "one sample, one shot" experiments to identify biomarker candidates for various diseases of the hepatobiliary system. In detail, sixteen different protocols with modifications at the stages of desalting, delipidation, deglycosylation and tryptic digestion were examined. The article states “For delipidation, the Cleanascite™ Lipid Removal Reagent and Clarification Kit (BSG, NJ 08852, USA) was used following manufacturer's instructions.”. The authors concluded that delipidation yielded a considerable number of complementary protein identifications and that Cleanascite™ treatment was indispensable for in-solution digestion methods. 

I am very pleased we now have many references for Cleanascite™ demonstrating its effectiveness to very selectively remove lipids from bile fluid, as this is will become a very important proximal body fluid for biomarker screenings in disease studies.” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at

About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for these expanding markets. Following a tiered business strategy, the company continues its growth in the consumable research products area supporting the rapidly expanding installation of LC-MS instrument and computational infrastructure. For this market, key products include: AlbuVoid™ and AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal, and NuGel™ for functional proteomics. From these innovations, the company has acquired knowledgebase and biomarker intellectual property assets that support discoveries of protein markers from blood, with special emphasis on early detection and personalized medical decisions for cancer patients. For more information, go to


Matthew Kuruc 732-274-2866