Class Specific Enrichment


KinaSorb™

Kinase (& ATP binding proteins) enrichment reagent

  • Reversible immobilization of phosphate group with optimal nucleotide orientation & specificity
  • Enrichment 3-5X, binding protein recoverable, ~200 μg
  • 60 minute, scaleable protocol compatible with functional assays, electrophoresis and LC-MS
  • Phosphatase activity & cyclic nucleotide phosphodiesterase (PDE) activity not detectable
  • Improves protein normalization when comparing heterogeneous tissues
  • On-bead digestion protocols for LC-MS IDs
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KinaSorb™ was used to enrich for both a narrow spectrum substrate profile – Hexokinase activity, and a broad spectrum protein kinase activity. The number of observable features was consistent with such narrow and broad spectrum activities.

2DE Comparison

Protein Kinase Activity of Beef Liver after KinaSorb™ Enrichment
Above: PEP Functional Activity Analysis, courtesy of ArrayBridge (St. Louis, MO), was employed for size separation and electro-eluted into the wells. Each well was monitored for Kinase activity using the ADP-Glo kit (Promega).

2DE Comparison

Hexokinase Activity after KinaSorb™ Treatment of Beef Liver
PEP Functional Activity Analysis, courtesy of ArrayBridge (St. Louis, MO), was employed for size separation and electro-eluted into the wells. Each well was monitored for Hexokinase activity using beef extract for cascade enzymes; NADP reduction being the final reporting measurement.

NuGel™ PBA
and
NuGel™ PBA Kit

Glycoprotein Enrichment Using Phenyl Boronic Acid

  • Enriches heterogeneous sets of glycoprotein’s, N-linked & O-linked
  • Consumable, no column regeneration
  • Species and tissue agnostic
  • Sorbitol elution; compatible with functional assays, electrophoresis and LC-MS
  • Binds biomolecules containing 1,2 cis-diol groups
  • Chemically derived, ideal for glyco-proteomic applications
  • NuGel™ polymer coating, porous silica based
  • Supplied as bead only (dry powder) or as kit (includes all binding and elution buffers)
Sample Type %Glycoprotein (Sorbitol Elution)
Mouse Plasma 33
Rat Serum 44
Sheep Serum 18
Bovine Serum 40
Bovine Brain Homogenate 9
View NuGel™ PBA View NuGel™ PBA Kit

Viraffinity™
and
ViraPrep™ Mammal Kit

Virus Enrichment & Purification

  • Purifies whole infectious non-enveloped virus, isolates antigenic virions
  • Enriches for viral proteins and nucleic acids
  • Use with (proven 350X enrichment) or without ultracentrifugation
  • Used for monitoring vaccine immune response
View Viraffinity™ View ViraPrep™ Mammal Kit

Our BSG Advantage


Consumable

Cost-effective, not derived from biologicals

Cost-effective, not derived from biologicals

  • No specialized instruments or HPLC
  • Economical surface chemistries, not derived from biologicals
  • No regeneration, so no prep to prep variability
  • Simple, fast microfuge bind/wash/elute protocols

On-Bead Digestion

Efficient workflows, quality LC-MS/MS data

Efficient workflows, quality LC-MS/MS data

  • Simple, reproducible workflows
  • Equivalent or better than in-solution digestion
  • Seamless to LC-MS, no desalting or C18 separations
  • Unique proteolytic efficiencies

Enrichment/Depletion

Diverse strategies, species agnostic

Diverse strategies, species agnostic

  • Products support strategies for both enrichment of low abundance proteomes, or depletion of high abundance proteins
  • Species agnostic not derived from biologicals

Functional Integrity

Maintained throughout all separations

Maintained throughout all separation

  • Mild buffer conditions maintains native structure with retained enzymatic, functional & bio-activities
  • Supports enzyme biomarker assays
  • Functional & Chemical Proteomics
  • Structural & activity-probe Proteomics
  • Top-down & ArrayBridge PEP Proteomics