Specific Enrichment - Multiple Applications
KinaSorb™
Kinase (& ATP binding proteins) enrichment reagent
- Reversible immobilization of phosphate group with optimal nucleotide orientation & specificity
- Enrichment 3-5X, binding protein recoverable, ~200 μg
- 60 minute, scaleable protocol compatible with functional assays, electrophoresis and LC-MS
- Phosphatase activity & cyclic nucleotide phosphodiesterase (PDE) activity not detectable
- Improves protein normalization when comparing heterogeneous tissues
- On-bead digestion protocols for LC-MS IDs
KinaSorb™ was used to enrich for both a narrow spectrum substrate profile – Hexokinase activity, and a broad spectrum protein kinase activity. The number of observable features was consistent with such narrow and broad spectrum activities.
Protein Kinase Activity of Beef Liver after KinaSorb™ Enrichment
Above: PEP Functional Activity Analysis, courtesy of ArrayBridge (St. Louis, MO), was employed for size separation and electro-eluted into the wells. Each well was monitored for Kinase activity using the ADP-Glo kit (Promega).
Hexokinase Activity after KinaSorb™ Treatment of Beef Liver
PEP Functional Activity Analysis, courtesy of ArrayBridge (St. Louis, MO), was employed for size separation and electro-eluted into the wells. Each well was monitored for Hexokinase activity using beef extract for cascade enzymes; NADP reduction being the final reporting measurement.
NuGel™ PBA
and
NuGel™ PBA Kit
and
Glycoprotein Enrichment Using Phenyl Boronic Acid
- Enriches heterogeneous sets of glycoprotein’s, N-linked & O-linked
- Consumable, no column regeneration
- Species and tissue agnostic
- Sorbitol elution; compatible with functional assays, electrophoresis and LC-MS
- Binds biomolecules containing 1,2 cis-diol groups
- Chemically derived, ideal for glyco-proteomic applications
- NuGel™ polymer coating, porous silica based
- Supplied as bead only (dry powder) or as kit (includes all binding and elution buffers)
| Sample Type | %Glycoprotein (Sorbitol Elution) |
|---|---|
| Mouse Plasma | 33 |
| Rat Serum | 44 |
| Sheep Serum | 18 |
| Bovine Serum | 40 |
| Bovine Brain Homogenate | 9 |
Viraffinity™
and
ViraPrep™ Mammal Kit
and
Virus Enrichment & Purification
- Purifies whole infectious non-enveloped virus, isolates antigenic virions
- Enriches for viral proteins and nucleic acids
- Use with (proven 350X enrichment) or without ultracentrifugation
- Used for monitoring vaccine immune response
BindPro™
and
NuGel™ BindPro™
and
Aqueous Protein Crash & Enrichment of Metabolites & Analytes
- Serum and plasma protein removal, >95%
- Aqueous protein crash
- < 30 minute protocol
- Applicable for target analytes, drug binding/screening and metabolomics
- Supplied as suspension reagent or NuGel™ dry powder format
Lane 1: Plasma Control
Lane 2: Plasma after treatment with BindPro™ Metabolomics.
Note: Protein bands are not present. Indicated quantitative protein binding to BindPro™ Metabolomics surface.
Lane 3: Eluent from BindPro™ Metabolomics.
Note: If necessary, proteins bound can be quantitatively recovered from BindPro™ Metabolomics.
Our BSG Advantage
Consumable
Cost-effective, not derived from biologicals
- No specialized instruments or HPLC
- Economical surface chemistries, not derived from biologicals
- No regeneration, so no prep to prep variability
- Simple, fast microfuge bind/wash/elute protocols
On-Bead Digestion
Efficient workflows, quality LC-MS/MS data
- Simple, reproducible workflows
- Equivalent or better than in-solution digestion
- Seamless to LC-MS, no desalting or C18 separations
- Unique proteolytic efficiencies
Enrichment/Depletion
Diverse strategies, species agnostic
- Products support strategies for both enrichment of low abundance proteomes, or depletion of high abundance proteins
- Species agnostic not derived from biologicals
Functional Integrity
Maintained throughout all separations
- Mild buffer conditions maintains native structure with retained enzymatic, functional & bio-activities
- Supports enzyme biomarker assays
- Functional & Chemical Proteomics
- Structural & activity-probe Proteomics
- Top-down & ArrayBridge PEP Proteomics