Selectionof AlbuVoid™ References:
Secretomefrom Cell Culture (BSA Removal)
Paulsen,Bruna, et al. " Reproducibledifferentiation of pure ovarian support cells from clinical-gradehiPSCs as a novel infertility treatment ." bioRxiv (2024):2024-04. To gain insight into the potential mechanism of actionof these cells during oocyte maturation beyond transcriptomicsreadouts, the investigators performed secretome proteomics toinvestigate proteins that overexpressed in OSC after 24 hours invitro in comparison with OSCs prior to culture (0 hour) from cellsderived, from both CG-hiPSC and RUO-hiPSC. For proteomic analysis,the article states, “The conditionedmedia …and then passed through albumin depletion columns (AlbuVoid) toeliminate HSA-derived albumin. ”.
Jenull,Sabrina, et al. "The histone chaperone HIR maintains chromatinstates to control nitrogen assimilation and fungal virulence." CellReports 36.3(2021): https://www.sciencedirect.com/science/article/pii/S2211124721008196#mmc1. Theauthors report a pivotal role for the HIR histone chaperone complexin modulating virulence of the human fungal pathogen Candidaalbicans . Thearticle states for “Cell-free supernatants from 16 hours YNB-BSA(0.025% BSA) cultures grown at 30°C were used for Mass-Specanalysis. Collected supernatants were lyophilized and dissolved in400 μl of water for AlbuVoid™ treatment for albumin depletion…Albumin-freeenriched secretory proteome was eluted from beads”.
Serum/Plasma
Paulsen et al.,Developmentof human induced pluripotent stem cell-derived ovarian support cellsas a clinical-grade product for in vitro fertilization,Cell Stem Cell (2025), https://doi.org/10.1016/j.stem.2025.12.020. AlbuVoid™ enrichment,helped in characterizing key functionalities of human inducedpluripotent stem cell (hiPSC) -derived cell lines.
Golden,Lesley R., et al. "APOE4to APOE2 allelic switching in mice improves Alzheimer’sdisease-related metabolic signatures, neuropathology andcognition ." NatureNeuroscience (2025):1-15. ApoE enrichment with AlbuVoid™,helped in identifying the closely related ApoE4/2 variants bytargeted plasma proteomic analysis.
Sae-Lee,Wisath, et al. " Theprotein organization of a red blood cell ." CellReports 40.3(2022): 111103. For plasma preparation, “Plasma was centrifuged at5,000 x g for 15 mins at 4°C, and only the top part of supernatantwas collected. The supernatantwas treated with AlbuVoid (Biotech Support Group) to reduce thepresence of serum albumin .”
Vialaret,Jerome & Kadi, Sarah & Tiers, Laurent & O Flynn, Robin &Lehmann, Sylvain & Hirtz, Christophe. (2018). Albumin depletionof human serum to improve quantitative clinical proteomics. CurrentTopics in Peptide & Protein Research 19.53-62. http://www.researchtrends.net/tia/abstract.asp?in=0&vn=19&tid=26&aid=6192&pub=2018&type=3
Inthis work, the investigators focused on depleting albumin from humanserum samples using an albumin depletion and low abundance proteinenrichment kit – AlbuVoid™, which enabled the detection ofseveral low-abundance proteins. By employing an optimized protocol,enriched proteins known as biomarkers for various diseases wereidentified. The authors concluded that the AlbuVoid™depletion method proved to be faster and more cost-effective thanantibody based methods ,and could be helpful for biomarker enrichment and detection inmedical research.
Jing,Lun, et al. " PROTEOMICANALYSIS IDENTIFIED LBP AND CD14 AS KEY PROTEINS IN BLOOD/BIPHASICCALCIUM PHOSPHATE MICROPARTICLE INTERACTIONS ." ActaBiomaterialia (2021). Here,in a LC-MS/MS proteomic study, thearticle describes use of HemoVoid™ and AlbuVoid™ prior to LC-MS analysis,“…After albumindepletion ,analysis of the significant deregulated proteins showed that 27signaling pathways significantly changed in blood cells… ”
Poillet-Perez,Laura, et al. "Autophagymaintains tumour growth through circulating arginine ."Nature (2018):1.
One invivo modelto study autophagy is whole-body deletion of the essential autophagygene Atg7 inadult mice which causes a systemic metabolic defect that manifests asstarvation intolerance. In order to measure the systemic proteomicresponse of such deletion in this study, AlbuVoid™was chosen as one method to remove albumin from mice and enrich thelow abundance proteomes from serum.
ZhengH, Zhao C, Qian M, Roy S, Arpa A, et al. (2015) AlbuVoid™Coupled to On-Bead Digestion – Tackling the Challenges of SerumProteomics. J Proteomics Bioinform 8: 225-230. The AlbuVoid™ bead enrichment is combined with a direct andseamless integration with Trypsin digestion, a method conventionallyreferred to as on-bead digestion. The digest time was evaluated as aparameter to identify whether different sub-populations of peptidesand proteins can be observed by LC-MS analyses. Using 2 differentdigestion times – 4 hours, and overnight, each with a singular 3hour gradient LC-MS run, between 400-500 total proteins were observedfor both human and rat sera, with overlapping and distinctsub-populations observable at each digest time. Theseresults support that the described methods gain workflow efficienciesover other high abundance depletion and in-solution digestionworkflows .
SwapanRoy, Matthew Kuruc. TheFunctional Subproteomes of Serpin Protease Inhibitors are Now Openfor LC-MS Biomarker Discovery .MOJ Proteomics Bioinform 2016, 3(6). Using bead-based separationprovided by the NuGel™ family of proteomic enrichment products -notably AlbuVoid™& AlbuSorb™, the authors demonstrate their utility to satisfyinvestigations of serum SERPINs. Theauthors also suggest their use to develop functional profiles of theSERPIN proteoforms, and how those can establish relationships todisease phenotypes, gene variant, and dysregulated mechanisms.
DavidL. Wang, Chuanguang Xiao, Guofeng Fu, Xing Wang and Liang Li.“ Identificationof potential serum biomarkers for breast cancer using a functionalproteomics technology ”.Biomarker Research (2017) 5:11.
Thearticle states “The mostdramatic difference for enzyme activity detection in usingthe AlbuVoid™ for serum protein enrichment wasdemonstrated in the case ofprotease activity analysis . Compared with the direct serum proteinase measurement, both thelevels and species of proteases were increased significantly in theenriched serum sample. …, andit is necessary to use AlbuVoid™ to enrich these lowlevel proteases to bring them to a high enough level to be detected .”
Fora full list of Albumin Removal references, visit: https://www.biotechsupportgroup.com/References-s/138.htm#albumin-depletion
