AlbuTrial™ Kit - 1 gram AlbuSorb™ Kit & 5 Preps AlbuVoid Kit

Trial-format kit for evaluating high abundance removal.
Species Agnostic
Suitable for LCMS, ELISA, Western Blot, Functional Analysis
Retains function and bio-activity

Product Code: AVS

AlbuTrial Kit

AlbuSorb™
Albumin Depletion From Serum or Plasma

Removes 30 mg albumin/ml, >90%
Affinity-type equivalence, virtually no cross-reactivity with other proteins
Disposable, no column regeneration or cross-contamination
Economical new surface technology, not based on blue-dye or immuno-affinity chromatography
Mild binding conditions maintains tertiary structure and simple transfer to secondary analysis
The flow-through (unbound) fractions retain their enzymatic and biological activity
Species agnostic

AlbuVoid™
Albumin Depletion Plus Low Abundance Serum Proteome Enrichment

Albumin voids out in flow-through (unbound) fraction >95%
Low abundance enrichment better than hexa-peptides or immune-affinity
Disposable, no column regeneration or cross-contamination
No low molecular weight or pI bias
Mild elution conditions maintains tertiary structure and simple transfer to secondary analysis
The elution fraction retain their enzymatic and biological activity
Optional on-bead digestion improves workflow and provides unique proteolytic efficiencies
Species agnostic

Click Here To View AlbuTrial™ Product Sheet

The high abundance of albumin in serum represses the signals for low abundance proteins, thereby interfering with the resolution and sensitivity of many protein profiling techniques. BSG refines the albumin depletion protocols establishing two albumin removal methods using chemical based proteome fractionation systems. The matrices AlbuSorb™ and AlbuVoid™ were tested on human, sheep, bovine, mouse, goat, rat, and calf from serum and plasma using a simple protocol. AlbuSorb™, facilities binding of albumin (>90%) thereby recovering the proteins in the flow-through. AlbuVoid™, by contrast, voids out the majority of albumin (>90%) in the flow-through and wash fractions. In the final elution step the low abundance proteins are enriched.AlbuTrial kit™ includes 1 gm of AlbuSorb™ and 5 Preps of AlbuVoid™ with respective buffers.

Product	Size	Quantity of Serum Processed	Item No.
AlbuTrial Kit™	5 PrepsAlbuVoid™and 1 gram ofAlbuSorb™	1 gram of AlbuSorb™ processes20 preps (25 µl serum) and 5Preps of AlbuVoid™ (processes200 µl serum)	AVS-05

AlbuSorb™ References
Cerebrospinal Fluid
Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270
Synovial fluid
Happonen KE, Fürst CM, Saxne T et al. PRELP protein inhibits the formation of the complement membrane attack complex.Journal of Biological Chemistry.2012;287(11):8092-100
Serum
Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.

Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats Nutrition and Cancer.2011;63(2):227-33

Holmberg, Rebecka Apolipoprotein CIII and Ljungan virus in diabetes 2010. Doctoral Thesis

Lu Q, Zheng X, McIntosh T Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Analytical Chemistry.2009;81(21):8715-23
AlbuVoid™ References
Serum
Swapan Roy, Matthew Kuruc. The Functional Subproteomes of Serpin Protease Inhibitors are Now Open for LC-MS Biomarker Discovery. MOJ Proteomics Bioinform 2016, 3(6): 00106

Serum Profiling Making Mark on Predictive Medicine
Vicki Glaser.Genetic Engineering & Biotechnology News. 2011;31(7):1-55.
Patents
Narain, Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas. "INTERROGATORY CELL-BASED ASSAYS AND USES THEREOF." U.S. Patent No. 20,120,258,874. 11 Oct. 2012.

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Kit includes:

AlbuTrial Kit™ AVS-05 Includes
AlbuVoid™ Kit	AlbuSorb™ Kit
Reagent (For 5 preps)	Quantity	Reagent (For 28 preps)	Quantity
AlbuVoid™ AVK-5	0.25 gram	AlbuSorb™ A185-1	1 gram
Binding Buffer AVBB	4 ml	Binding Buffer BB1	30ml
Wash Buffer AVWB	5 ml
Elution Buffer AVEB	2 ml
Corning® Spin-X Filter	5
Note: Please contact [email protected]for prices in bulk amount.

MSDS

MSDS-AlbuSorb™, Catalog Order # A185-
MSDS-AlbuVoid™, Catalog Order # AVK-10, AVK-50
MSDS-AlbuVoid™ Binding Buffer AVBB
MSDS-AlbuVoid™ Elution Buffer AVEB
MSDS-AlbuVoid™ Wash Buffer AVWB

Click Here To View AlbuVoid™ PLUS Safety Data Sheet - AlbuVoid
Click Here To View AlbuVoid™ PLUS Safety Data Sheet - AVBB
Click Here To View AlbuVoid™ PLUS Safety Data Sheet - AVWB
Click Here To View AlbuVoid™ PLUS Safety Data Sheet - AVEB
Click Here To View AlbuVoid™ PLUS Safety Data Sheet - AlbuSorb
Click Here To View AlbuVoid™ PLUS Safety Data Sheet - BB1

Product Code	Name	Base Price	Qty
Product Code:AVS-05	AlbuTrial Kit - 1 gram AlbuSorb™ Kit & 5 Preps AlbuVoid Kit	Base Price:$685.00

AlbuTrial™ Kit - 1 gram AlbuSorb™ Kit & 5 Preps AlbuVoid Kit

Description