Sample Prep for Mass Spectrometry
- Proteome Enrichment
- Not based on immuno-affinity
- Bead Assisted Sample Prep (BASP™)
- LC-MS, MALDI Compatible Protocols
High abundance proteins and lipids are well known interferences in mass spectrometry based proteomic analysis. For years the protein depletion toolkit was limited primarily to immuno-affinity chromatography and other biologically-derived tools.
BSG offers two complementary technologies for proteomic researchers to selectively bind or enrich, in order to achieve the best results. By using consumable non-biologically derived beads, researchers will be able to improve efficiency and quantitation essential for expanding mass spectrometry into routine healthcare.
AlbuVoid™ LC-MS On-Bead
Albumin depletion plus low abundance protein enrichment, coupled with optimized on-bead digestion (BASP™) protocols for LC-MS serum and plasma proteomics
- Seamless workflows
- Unique proteolytic efficiencies
- Label, label free compatible
Total LC-MS/MS Proteins identified at 2 digestion times: 4 hours (4h) & overnight (O/N), single 3 hour LC gradient.
AlbuVoid™ PLUS
Albumin and IgG Depletion From Serum/Plasma for Proteomics
- IgG removal >90% (70-80% of total Immunoglobulins removed)
- Albumin removal >95%
- Seamless and simple < 1 hour protocol
- Low abundance enrichment equivalent to immuno-affinity
- Disposable, cost-effective, no column regeneration or cross-contamination
- Works for most species tested including human, sheep, rat, mouse, bovine
- On-bead protocols improve workflow and efficiency, especially suited to targeted proteomics
- Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional Assays.
20µg of protein loaded on to 10% tris bis gel
A - Serum alone – shows IgG and Albumin regions
B - NuGel™ ProteinA treated serum – shows IgG depletion
C - AlbuVoid™ treated serum – shows Albumin depletion
D - AlbuVoid™ PLUS treated serum – shows IgG and Albumin depletion
HemoVoid™ LC-MS On-Bead
Hemoglobin depletion plus low abundance protein enrichment, coupled with optimized on-bead digestion protocols (BASP™) for LC-MS erythrocyte & blood proteomics
- Seamless workflows
- Unique proteolytic efficiencies
- Label, label free compatible
AlbuSorb™
Selectively Binds Albumin
- Removes serum albumin >90%
- Economical small ligand surface architecture (not dye-based), bio-affinity performance
- Species agnostic
AlbuSorb™ PLUS
Selectively Binds Albumin & Immunoglobulin
- > 85% Albumin, 85% IgG depleted from 25 μl serum
Quantitative Bias Comparison between
AlbuSorb™ & AlbuSorb™ PLUS
| AlbuSorb™ | AlbuSorb™ PLUS | |
|---|---|---|
| Total IG IDs 2+ Spectral Cts | 80 | 58 |
| Total IG % of Spectral Count | 24% | 9% |
| Total Albumin % of Spectral Count | 12% | 15% |
Reference
For serum exosome enrichment
HemogloBind™
and
NuGel™ HemogloBind™
Removes Hemoglobin Interference
- Highly specific for hemoglobin binding
- depletion from hemolyzed serum, dura, BALF, and whole blood
- supports biomarker quantitative analysis
Cleanascite™
Lipid Adsorption & Clarification
- Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon)
- Workflows for antibodies, proteins, nucleic acids, proteoglycans, and most serum analytes
- A high binding capacity for lipids with minimal cross-reactivity with proteins and nucleic acids
- Ideal for clarifying ascites, serum, cell & tissue culture, bile and organ homogenates
Cleanascite™ is supplied as a suspension reagent. Simply add, mix and centrifuge in a 10 minute protocol.
References
Plasma/Serum Protein Biomarkers
For diabetes biomarkers, Cleanascite™ is shown both to improve LC-MS measurements, and validated in accordance with CLIA ’88 guidelines. { doi: 10.1016/j.cca.2016.01.019 }
Bile Proteomics
Cleanascite™ treatment was indispensable for in-solution digestion methods. { https://pubag.nal.usda.gov/catalog/5599484 }
For MALDI-TOF Analysis
Farina A, Dumonceau JM, Frossard JL. Proteomic Analysis of Human Bile from Malignant Biliary Stenosis Induced by Pancreatic Cancer Journal of Proteome Research.2009; 8(1):159-69
NRicher™ Kits
Proteome Enrichment Simplified & Diversified
NRicher™ Mx
General Enrichment for All Biofluids and Tissue Lysates
- Consumable chemically derived beads, species agnostic as they are not derived from antibodies
- Enrich low abundance proteomes from any source, from sera/plasma to cell lysates from both animals and humans, >90% Albumin removal
- Does not require any specialized instruments, just a standard microfuge
- Bead format suitable for automation compatibility, please inquire
- On-Bead digestion for LC-MS analysis, or optional elution for any functional, enzymatic, or immunoassay analysis
NRicher™ Apo
Enrichment of Apolipoproteins
- Consumable chemically derived beads, species agnostic as they are not derived from antibodies
- Enrich Apolipoproteins from sera or plasma from both animals and humans, >90% Albumin removal
- Does not require any specialized instruments, just a standard microfuge
- Bead format suitable for automation compatibility, please inquire
- On-Bead digestion for LC-MS analysis, or optional elution for any functional, enzymatic, or immunoassay analysis
NRicher™ Ig
Enrichment of all isotypes and subclasses of Immunoglobulins
- Consumable chemically derived beads, species agnostic as they are not derived from antibodies
- Enrich circulating immune complexes from sera or plasma from both animals and humans, >90% Albumin removal
- Does not require any specialized instruments, just a standard microfuge
- Bead format suitable for automation compatibility, please inquire
- On-Bead digestion for LC-MS analysis, or optional elution for any functional, enzymatic, or immunoassay analysis
NRicher™ C
Enrichment of Complement Cascade Related Proteins
- Consumable chemically derived beads, species agnostic as they are not derived from antibodies
- Enrich Complement Cascade related proteins from sera/plasma or cell lysates from both animals and humans, >90% Albumin removal
- Does not require any specialized instruments, just a standard microfuge
- Bead format suitable for automation compatibility, please inquire
- On-Bead digestion for LC-MS analysis, or optional elution for any functional, enzymatic, or immunoassay analysis
Our BSG Advantage
Consumable
Cost-effective, not derived from biologicals
- No specialized instruments or HPLC
- Economical surface chemistries, not derived from biologicals
- No regeneration, so no prep to prep variability
- Simple, fast microfuge bind/wash/elute protocols
On-Bead Digestion
Efficient workflows, quality LC-MS/MS data
- Simple, reproducible workflows
- Equivalent or better than in-solution digestion
- Seamless to LC-MS, no desalting or C18 separations
- Unique proteolytic efficiencies
Enrichment/Depletion
Diverse strategies, species agnostic
- Products support strategies for both enrichment of low abundance proteomes, or depletion of high abundance proteins
- Species agnostic not derived from biologicals
Functional Integrity
Maintained throughout all separations
- Mild buffer conditions maintains native structure with retained enzymatic, functional & bio-activities
- Supports enzyme biomarker assays
- Functional & Chemical Proteomics
- Structural & activity-probe Proteomics
- Top-down & ArrayBridge PEP Proteomics