Sample Prep for Mass Spectrometry
High abundance proteins and lipids are well known interferences in mass spectrometry based proteomic analysis. For years the protein depletion toolkit was limited primarily to immuno-affinity chromatography and other biologically-derived tools. BSG offers two complementary technologies for proteomic researchers to selectively bind or enrich, in order to achieve the best results. By using consumable non-biologically derived beads, researchers will be able to improve efficiency and quantitation essential for expanding mass spectrometry into routine healthcare.
|
| AlbuSorb™ | AlbuSorb™ PLUS | |
|---|---|---|
| Total IG IDs 2+ Spectral Cts | 80 | 58 |
| Total IG % of Spectral Count | 24% | 9% |
| Total Albumin % of Spectral Count | 12% | 15% |
Reference
For serum exosome enrichment
HemogloBind™
and
NuGel™ HemogloBind™
and
Removes Hemoglobin Interference
- Highly specific for hemoglobin binding
- depletion from hemolyzed serum, dura, BALF, and whole blood
- supports biomarker quantitative analysis
Cleanascite™
Lipid Adsorption & Clarification
- Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon)
- Workflows for antibodies, proteins, nucleic acids, proteoglycans, and most serum analytes
- A high binding capacity for lipids with minimal cross-reactivity with proteins and nucleic acids
- Ideal for clarifying ascites, serum, cell & tissue culture, bile and organ homogenates
Cleanascite™ is supplied as a suspension reagent. Simply add, mix and centrifuge in a 10 minute protocol.
References
Plasma/Serum Protein Biomarkers
For diabetes biomarkers, Cleanascite™ is shown both to improve LC-MS measurements, and validated in accordance with CLIA ’88 guidelines. { doi: 10.1016/j.cca.2016.01.019 }
Bile Proteomics
Cleanascite™ treatment was indispensable for in-solution digestion methods. { https://pubag.nal.usda.gov/catalog/5599484 }
For MALDI-TOF Analysis
Farina A, Dumonceau JM, Frossard JL. Proteomic Analysis of Human Bile from Malignant Biliary Stenosis Induced by Pancreatic Cancer Journal of Proteome Research.2009; 8(1):159-69
NuGel™ NRicher™ 6
Top-down proteomics enrichment kit
- 12 differentiated subproteomes, 6 flow-through fractions, and 6 elution fractions
- Enrich low abundance functional biomarkers for sequence and structural annotation
- In a rigorous examination of protein complexes, about twice the number of observations were made possible through sub-proteome bias characteristics of NRicher™ 6 { Nature doi:10.1038/nature14877 }
- Kit includes 6 mixed mode bead chemistries per prep
- All Top-down proteomic applications
- Optimize biomarker enrichment after initial enrichment using NRicher™ Mx
LC-MS Protein Identifications
NRicher™ 6 Improved dynamic range and ID coverage by sub-proteome capture Nature doi:10.1038/nature14877
NuGel™ NRicher™ Mx
Low Abundance Proteome Enrichment
- Enriches & normalizes sub-proteomes
- Compress proteome concentrations
- Species and tissue agnostic
- Composite of the NRicher™ 6 mixed mode beads
Non-reduced SDS-PAGE profiles show that major high abundance bands are greatly reduced. The low abundance bands are enriched relative to their high abundance bands, visually estimated to be 5-10X for most bands. Because of its heterogeneity, the IgG variants co-localize to the same region.
Our BSG Advantage
Consumable
Cost-effective, not derived from biologicals
- No specialized instruments or HPLC
- Economical surface chemistries, not derived from biologicals
- No regeneration, so no prep to prep variability
- Simple, fast microfuge bind/wash/elute protocols
On-Bead Digestion
Efficient workflows, quality LC-MS/MS data
- Simple, reproducible workflows
- Equivalent or better than in-solution digestion
- Seamless to LC-MS, no desalting or C18 separations
- Unique proteolytic efficiencies
Enrichment/Depletion
Diverse strategies, species agnostic
- Products support strategies for both enrichment of low abundance proteomes, or depletion of high abundance proteins
- Species agnostic not derived from biologicals
Functional Integrity
Maintained throughout all separations
- Mild buffer conditions maintains native structure with retained enzymatic, functional & bio-activities
- Supports enzyme biomarker assays
- Functional & Chemical Proteomics
- Structural & activity-probe Proteomics
- Top-down & ArrayBridge PEP Proteomics