NewsRelease
NatureArticle Cites HemogloBind™ and NRicher™ for MacromolecularComplex Annotation
MONMOUTHJUNCTION, NJ, February9, 2016 -- Biotech Support Group reports on a recent research articlepublished in Nature, describing the simplicity and efficiency oftheir proteomic enrichment technology to observe and annotate solublemultiprotein complexes common to metazoa.
Thecitation is:
CWan, BBorgeson, SPhanse, F Tu, KDrew, GClark, et al. Panoramaof ancient metazoan macromolecular complexes.NatureVolume:525,Pages:339–344Datepublished:(17September 2015).
DOI:doi:10.1038/nature14877
Inbrief, the article’s authors aimed at elucidatingthe components of multiprotein complexes on a proteome-wide scale.The authors identify protein complexes from nine species in parallel,based on biochemical fractionation of native soluble macromolecularcomplexes followed by tandem mass spectrometry to identifycomponents. The article states “…clarified heart and liverhomogenates were frozen, and subsequently pooled, depleted ofhemoglobin using HemogloBind™ according to manufacturer’sinstructions…prior to sucrose gradient fractionation. A secondBiotech Support Group product, formerly called SeraFILE PROspectorand now trademarked as NRicher™ 6, was used for sub-proteomeenrichment. The article states “Affinity bead based samplepre-separations were performed for C.eleganslysates as per manufacturer’s instructions…fractions (SeraFILEbead eluates) were subjected to ion exchange fractionation…”. Theauthors conclude that many complexes are conserved across species,and by correlating the results with genome sequence information, areable to predict more than one million interactions in 122 eukaryotes.
“Iam very pleased that two of our products were able to contribute tothis rigorous examination of protein complexes. When our productswere used as a pretreatment step in the overall workflow, about twicethe number of observations and annotations became possible. Thisfurther validates that the sub-proteome bias characteristics ofNRicher™ 6 can simplify complex proteomes into less complexsub-proteomes with efficiencies suitable for deep functional proteomecharacterization. Furthermore, this study demonstrated the importanceof a key feature implicit to all of our products; that is themaintenance of functional and structural integrity after separations.Without that particular feature, these additional observations wouldnot have been possible.” states Swapan Roy, Ph.D., President andFounder of Biotech Support Group.
Formore information on HemogloBind™, visit:
http://www.biotechsupportgroup.com/HemogloBind-Hemoglobin-Depletion-From-Hemolyzed-p/h0145.htm
Formore information on NRicher™ 6, visit:
http://www.biotechsupportgroup.com/NuGel-NRicher-6-p/sr610.htm
About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of proteomic, metabolomic and genomic sample preparation and enrichment products and services. It’s principal products include: AlbuVoid™ & AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ for nucleic acid isolation. For more information, go to http://www.biotechsupportgroup.com
Contact:
Dr. Swapan Roy & Matthew Kuruc
Biotech Support Group LLC
1 Deer Park Drive, Suite M
Monmouth Junction NJ 08852
732-274-2866 Worldwide
800-935-0628 North America
[email protected]
