Hemovoid™ Removes Hemoglobin From Dried Blood Spot (Blood Card) For Protein Enrichment

MONMOUTH JUNCTION, NJ.– Pharmaceutical companies and clinical laboratories are using dried blood spot (DBS) technology for newborn screening of metabolic disorders, epidemiological surveys (e.g. HIV monitoring), therapeutic drug monitoring (TDM) and toxicology analysis. Hemoglobin is a common contaminant in whole blood. Hemoglobin causes increased blood procoagulant activity which is thought to be a marker of macrophage activation. Spectrophotometric and other procedures are implemented to test for hemoglobin (Hb) contamination in tissues. HemoVoid™ is a silica based NuGel™ polyelectrolyte matrix and it removes hemoglobin from dried whole blood card. The HemoVoid™ protocol uses mild buffers and the protocol conditions are so gentle that native enzyme and other functional activity is retained in elution fractions. After implementing the HemoVoid™ Blood Card protocol it produces enriched proteins free of hemoglobin which are used for biomarkers and protein analysis. The hemoglobin enriched filtrate could have hemoglobin variants, hemoglobin binding proteins or other analytes optimal for biomarker studies. HemoVoid™ Blood Card protocol is compatible with validated LC-MS/MS assays.

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Advantages of Dried Blood Spot are:

  • Convenience: DBS does not require venipuncture, use of anticoagulant or plasma separation. Compatible with analytical approaches used by pharmaceutical companies and clinical laboratories allowing for automated handling of DBS samples without pretreatment.
  • Small sample volume: Venous sampling requires more volume.DBS volume is less than 20ul on filter paper. Dried blood spot (DBS) analysis has substantially reduced the volume of blood needed. Ethical benefits for preclinical studies are reduced volumes, removal of satellite requirement on short term toxicology studies and reduction of rodent warming.
  • Cost effective: DBS samples could be stored at room temperature without dry ice thus reducing cost of shipping and storage of filter papers.
  • Analyte stability : DBS samples stored for months to years are similar to plasma samples stored at -20 degrees C and provide greater metabolite stability.
  • Increased safety: Drying causes pathogens to deactivate on the filter paper and transfer of blood onto filter paper has low infection risk.

Characteristics of Hemovoid™

  • Hemoglobin voids in flow-through >98%, with <30 minute bind/wash/elute protocol.
  • Hemoglobin removal from whole blood lysates extracted from dried blood cards.
  • Hemoglobin removal from frozen and fresh blood.
  • Hemoglobin removal from erythrocytes (red blood cells).
  • Blood proteins and enzymes are enriched for potential biomarker and proteomic studies.
  • Disposable, cost-effective and high throughput hemoglobin depletion method.
  • Removes hemoglobin from whole blood of diverse species including human, mice, sheep, bovine, goat, rat, etc.
  • HemoVoid™ protocol is designed to substantially reduce the presence of hemoglobin and it’s associated interference with many serum protein analytes.

About Biotech Support Group LLC

Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include: AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, NuGel™ for passivated silica-based affinity chromatography, and ProCipitate™ & ProPrep™ for nucleic acid isolation. Currently, Biotech Support Group LLC and ProFACT Proteomics Inc., are collaborating on the development of a proteomics platform used in functional profiling for proteomic analysis and a separations method for generating sub-proteomes used in biomarker and functional proteomic prospecting. For more information, go to: www.biotechsupportgroup.com

Dr. Swapan Roy & Matthew Kuruc
Biotech Support Group LLC
1 Deer Park Drive, Suite M
Monmouth Junction NJ 08852
732-274-2866 Worldwide
800-935-0628 North America

Hemovoid™ References

Suggested References

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Posted on Date:
Sun, 07/22/2012