Latest Applications

Proteomics Research Article Cites HemogloBind™ in Profiling Lung Injury

In brief, the authors consider pulmonary complications due to infection and idiopathic pneumonia syndrome (IPS) in hematopoietic stem cell transplant (HSCT) recipients. For this, a proteomic characterization of global bronchoalveolar lavage fluid (BALF) was studied to identify proteins and pathways that differentiate IPS from infectious lung injury after HSCT. Because the BALF samples appeared tinged with blood, the authors tested HemogloBind™ to determine whether the removal of hemoglobin (in addition to high-abundance proteins) would improve the depth of coverage. The article states "Hemoglobin removal improved protein identification to 845 proteins at 1% FDR compared with 496 proteins with high-abundance protein depletion alone". The authors conclude that the protein expression differences provide insights into mechanisms activated in lung injury and suggest potential therapeutic targets to augment lung repair.

MOJ Proteomics & Bioinformatics Report - The Functional Subproteomes of SERPIN Protease Inhibitors Are Now Open For LC-MS Biomarker Discovery
Highlights the versatility of BSG's Albumin Removal Products

Conformational variants of the unique family of protease inhibitors annotated as SERPINs, are most often underrepresented in proteomic analyses. This limits understanding the complex regulation that this family of proteins presents to the networks within the protease web of interactions. Using bead-based separation provided by the NuGel™ family of proteomic enrichment products - notably AlbuVoid™ & AlbuSorb™, after albumin depletion, the authors demonstrate their utility to satisfy investigations of serum SERPINs. The authors also suggest their use to develop functional profiles of the SERPIN proteoforms, and how those can establish relationships to disease phenotypes, gene mutations, and dysregulated mechanisms.