News Release

Journal Article Cites HemoVoid™ in Nanopore Analysis of α-Synuclein Post-translational Modifications

MONMOUTH JUNCTION, NJ , October 16, 2023 -- Biotech Support Group reports on a research article describing the simplicity and efficiency of their HemoVoid™ technology for enriching erythrocyte low abundance proteins, in order to assess post-translational modifications of α-synuclein through a biological nanopore.

The citation is:

Cao, Chan, et al. " Deep learning-assisted single-molecule detection of protein post-translational modifications with a biological nanopore ." bioRxiv (2023): 2023-09.

Nanopore sensing provides high sensitivity for the detection of low abundance proteins, holding the potential to impact single-molecule proteomics and PTM detection in particular. Here, we demonstrate the ability of a biological nanopore, the pore forming toxin aerolysin, to detect and distinguish α-synuclein-derived peptides bearing single or multiple PTMs, namely phosphorylation, nitration and oxidation occurring at different positions and in various combinations. To deplete Hemoglobin, the article states “Briefly, RBCs were lysed and further treated using the HemoVoid kit (Biotech Support Group), aiming to remove hemoglobin (the most abundant protein in RBCs) but also to enrich low abundant proteins such as α-synuclein.” The study demonstrates a framework that can quantify α-synuclein peptides at picomolar concentration and detect the C-terminal peptides generated by digestion of full-length α-synuclein. Collectively, the work highlights the unique advantage of using nanopore as a tool for simultaneous detection of multiple PTMs and paves the way for their use in biomarker discovery and diagnostics.

“It’s very rewarding to see that for Hemoglobin removal, HemoVoid™ was an essential product to enrich the red blood cell proteome for this study. This is the second reference whereby HemoVoid™ is used to enrich for variants of α-synuclein. Our new product NRicher™ Mx also enriches for α-synuclein from serum”, states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

For more information on HemoVoid™, visit:

http://www.biotechsupportgroup.com/HemoVoid-Hemoglobin-Depletion-From-Erythrocytes-p/hvk.htm

For more information of all of our Hemoglobin removal products, visit:

https://www.biotechsupportgroup.com/Hemoglobin-Removal-s/312.htm

For more information on NRicher™ Mx visit:

https://www.biotechsupportgroup.com/product-p/nimx-10.htm

About Biotech Support Group LLC

Converging with cultural and technological disruptions forthcoming in healthcare, Biotech Support Group develops methods for cost effective and efficient sample prep essential for expanding proteomic analysis. Following a tiered business strategy, the company continues its growth in the consumable research products area. For this market, key products include: AlbuVoid™ and AlbuSorb™ PLUS for albumin & IgG depletion, Cleanascite ™ for lipid adsorption, HemogloBind™ and HemoVoid™ for hemoglobin removal, and NRicher™ for low abundance and family specific proteome enrichment. For more information, go to http://www.biotechsupportgroup.com

For business development contact: Matthew Kuruc
732-274-2866 mkuruc@biotechsupportgroup.com

Keywords

α-synuclein, Hemoglobin depletion, erythrocyte proteomics, Parkinson’s disease, Biological nanopores