KinaSorb™ was used to enrich for both a narrow spectrum substrate profile – Hexokinase activity, and a broad spectrum protein kinase activity. The number of observable features was consistent with such narrow and broad spectrum activities.

Protein Kinase Activity of Beef Liver after KinaSorb™ Enrichment
Above: PEP Functional Activity Analysis, courtesy of ArrayBridge (St. Louis, MO), was employed for size separation and electro-eluted into the wells. Each well was monitored for Kinase activity using the ADP-Glo kit (Promega).

Hexokinase Activity after KinaSorb™ Treatment of Beef Liver
PEP Functional Activity Analysis, courtesy of ArrayBridge (St. Louis, MO), was employed for size separation and electro-eluted into the wells. Each well was monitored for Hexokinase activity using beef extract for cascade enzymes; NADP reduction being the final reporting measurement.