Sepax Zenix™ SEC Column

Particle size of 3 µm
Selection of pore size: 100, 150 and 300 Å
Highest separation efficiency and resolution
High capacity
High stability over low and high concentration salt
Lot-to-lot reproducibility
High protein recovery with intact biological activity
Negligible non-specific interactions
Ideal for separation and analysis of biological molecules: proteins, nucleic acids, oligonucleotides, peptides and virus
Ideal for separation and analysis of natural polymers, e.g. polysaccharides, synthetic polymers, and nanomaterials, e.g. nanoparticles

Zenix SEC 3µm phases are made of uniform, hydrophilic, and neutral nanometer thick proprietary surface coating chemically bonded on high purity and mechanically stabilized silica. Zenix SEC 3µm unique packing combined with large pore volume delivers highest and unrivaled separation efficiency and resolution. The well-controlled surface chemistry results in excellent lot-to-lot reproducibility. Our unique bonding chemistry, coupled with the maximized bonding density, allows Zenix SEC to provide high stability and negligible non-specific interactions. The available pore sizes of Zenix packings are 100, 150 and 300Å. Typical applications for Zenix SEC columns include separation and analysis of biological molecules and water soluble polymers in aqueous buffers.

Zenix SEC Technical Specifications

Phase	Zenix SEC-80	Zenix SEC-100	Zenix SEC-150	Zenix SEC-300
Material	Neutral, hydrophilic film bonded silica
Particle size	3 µm
Pore size	~ 80 Å	~ 100 Å	~ 150 Å	~ 300 Å
Protein MW range (native)	100 - 50,000	100 - 100,000	500 - 150,000	5,000 - 1,250,000
pH stability	2 - 8.5 (pH 8.5-9.5 can be tolerated temporarily.)
Backpressure (psi for a 7.8×300 mm)	~ 1,500	~ 1,500	~ 1,500	~ 1,500
Maximum backpressure (psi)	~ 4,500	~ 4,500	~ 4,500	~ 3,500
Salt concentration range	20 mM - 2.0 M
Maximum temperature (°C)	~ 80
Mobile phase compatibility	Aqueous and organic