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HemogloBind™ Used for Hemoglobin Depletion in Site-Specific GlcNAcylation of Human Erythrocyte Proteins: Potential Biomarker(s) for Diabetes Mellitus study MONMOUTH JUNCTION, N.J. — HemogloBind™ is engineered for a high degree of hemoglobin binding selectivity and does not cross react with most common serum components. HemogloBind™’s binding capacity for hemoglobin is approximately 10mg/ml of hemoglobin. HemogloBind™ is used mostly on hemolysed serum.
Details about HemogloBind™ Research studies that mention HemogloBind™:
Kyoungsook Park, Christopher D. Saudek, and Gerald W. Hart (2010) Increased Expression of β-N-Acetylglucosamindase (O-GlcNAcase) in Erythrocytes from Prediabetic and Diabetic Individuals. Diabetes. 59(7):1845-50. O-GlcNAc actively cycles on erythrocyte, regulates insulin signaling and is a mediator of glucose toxicity. Therefore studying it may reveal potential biomarker for diagnoses of diabetes. Highly efficient enrichment methods based Hemoglobind overcome the challenges of low stoichiometry, suppressed ionization efficiency in presence of unmodified peptides, and intrinsic lability in gas phase mass spectrometric methods. In this paper, authors used Hemoglobind to study erythrocyte proteins and compared it with their abundance between normal and diabetic samples proteins. Blood samples were obtained from normal and diabetic patients collected into a vial containing EDTA and OGlcNAcase inhibitor PUGNAc. Next the researchers fractionated the blood cells to isolate erythrocytes. After erythrocytes were lysed and centrifuged, the supernatant containing hemoglobin was partially depleted by HemogloBind™ from Biotech Support Group
Biotech Support Group LLC is a leading developer of proteomic and genomic sample preparation and enrichment products. Its principal products include: AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ & ProPrep™ for nucleic acid isolation. For more information, go to www.biotechsupportgroup.com.
Dr. Swapan Roy & Matthew Kuruc |
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