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Research Article Reports The Use of HemogloBind™ in Research On Hemolytic Anemia in Mature Erythrocytes MONMOUTH JUNCTION, NJ — Biotech Support Group reports on a recent research article which supports the utility of their protein specific sample depletion consumable product for hemoglobin depletion. The citation is: Hikosaka, Keisuke, et al. “Deficiency of Nicotinamide Mononucleotide Adenylyltransferase 3 (Nmnat3) Causes Hemolytic Anemia by Altering the Glycolytic Flow in Mature Erythrocytes.” Journal of Biological Chemistry (2014):published online April 16, 2014. doi: 10.1074/jbc.M114.554378 Authors Hikosaka et al describe research on nicotinamide mononucleotide adenylyltransferase 3 (Nmnat3) from red blood cells and its regulation of nicotinamide adenine dinucleotide. Detection of hemolytic anemia and splenomegaly in addition to reduced adenosine triphosphate (ATP) levels was noted in cells not containing Nmnat3. Glycolysis progression was stalled at the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) step as a deficiency of Nmnat3 led to lack of co-enzyme NAD. LC-MS/MS metabolomics and stable isotope tracer analysis revealed the impact of reduced NAD quantity in RBCs on glycolysis and pentose phosphate pathway. For selective extraction of hemoglobin from erythrocytes, the article states “Each sample was normalized by hemoglobin concentration at 20 µg/µl, and then hemoglobin was depleted using HemogloBind”. “We continue to find new uses of HemogloBind™ in erythrocyte research as hemoglobin accounts for about 97% of the total protein mass, and in many cases needs to efficiently removed. This article further validates our unique surface technology approach, not based on antibodies or engineered bio-ligands, as being highly selective and an efficient method for the depletion of hemoglobin” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group. About Biotech Support Group LLC Biotech Support Group LLC is a leading developer of consumable proteomic, metabolomic and genomic sample preparation and enrichment products. It’s principal products include: AlbuVoid™ & AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ & ProPrep™ for nucleic acid isolation. For more information, go to http://www.biotechsupportgroup.com
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Posted on Date:
Sat, 05/03/2014
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