Obesity Research & Peroxynitrite Decomposition Catalysts Sample Preparation Using HemogloBind™ MONMOUTH JUNCTION, NJ – A research article in the journal Archives of Biochemistry & Biophysics (http://www.sciencedirect.com/science/article/pii/S0003986115001988) cites Biotech Support Group’s HemogloBind™ (http://www.biotechsupportgroup.com/node/75) sample preparation reagent to deplete hemoglobin (Hb) from lysed red blood cells. The article states: “Blood plasma (50 µl) was mixed with HemogloBind (50 µl) to remove hemoglobin from lysed red blood cells.” Plasma hemoglobin is depleted using Biotech Support Group’s HemogloBind™ to extract hemoglobin from lysed red blood cells. Plasma concentration of high-density lipoprotein (HDL), total cholesterol and triacylglycerol (TAG) is obtained. Precipitation using polyethylene glycol (PEG) of beta-lipoproteins such as very low-density lipoprotein (VLDL) and low-density lipoprotein(LDL) from HDL fractions is performed. “We are pleased with this data on peroxynitrite decomposition catalysts. HemogloBind™ minimizes hemoglobin interference from blood samples and allows research on cholesterol proteins” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
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Contact: Reference Johns, Michael, et al. “SR-135, a peroxynitrite decomposing catalyst, enhances β-cell function and survival in B6D2F1 mice fed a high fat diet.”Archives of Biochemistry and Biophysics (2015). Suggested References Ding, R., et al. “Peroxynitrite decomposition catalyst prevents matrix metalloproteinase-9 activation and neurovascular injury after hemoglobin injection into the caudate nucleus of rats.” Neuroscience 297 (2015): 182-193. Kosower, Nechama S., Rela L. Koppel, And Edward M. Kosower. “Sensitivity of hemoglobin thiol groups within red blood cells of rat during oxidation of glutathione.” European Journal of Biochemistry 77.3 (1977): 529-534. |

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