Article Cites HemogloBind™ and NRicher™ for Macromolecular
JUNCTION, NJ, February
9, 2016 -- Biotech Support Group reports on a recent research article
published in Nature, describing the simplicity and efficiency of
their proteomic enrichment technology to observe and annotate soluble
multiprotein complexes common to metazoa.
Phanse, F Tu, K
Clark, et al. Panorama
of ancient metazoan macromolecular complexes.
brief, the article’s authors aimed at elucidating
the components of multiprotein complexes on a proteome-wide scale.
The authors identify protein complexes from nine species in parallel,
based on biochemical fractionation of native soluble macromolecular
complexes followed by tandem mass spectrometry to identify
components. The article states “…clarified heart and liver
homogenates were frozen, and subsequently pooled, depleted of
hemoglobin using HemogloBind™ according to manufacturer’s
instructions…prior to sucrose gradient fractionation. A second
Biotech Support Group product, formerly called SeraFILE PROspector
and now trademarked as NRicher™ 6, was used for sub-proteome
enrichment. The article states “Affinity bead based sample
pre-separations were performed for C.
lysates as per manufacturer’s instructions…fractions (SeraFILE
bead eluates) were subjected to ion exchange fractionation…”. The
authors conclude that many complexes are conserved across species,
and by correlating the results with genome sequence information, are
able to predict more than one million interactions in 122 eukaryotes.
am very pleased that two of our products were able to contribute to
this rigorous examination of protein complexes. When our products
were used as a pretreatment step in the overall workflow, about twice
the number of observations and annotations became possible. This
further validates that the sub-proteome bias characteristics of
NRicher™ 6 can simplify complex proteomes into less complex
sub-proteomes with efficiencies suitable for deep functional proteome
characterization. Furthermore, this study demonstrated the importance
of a key feature implicit to all of our products; that is the
maintenance of functional and structural integrity after separations.
Without that particular feature, these additional observations would
not have been possible.” states Swapan Roy, Ph.D., President and
Founder of Biotech Support Group.
more information on HemogloBind™, visit:
more information on NRicher™ 6, visit:
About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of proteomic, metabolomic and genomic sample preparation and enrichment products and services. It’s principal products include: AlbuVoid™ & AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ for nucleic acid isolation. For more information, go to http://www.biotechsupportgroup.com
Dr. Swapan Roy & Matthew Kuruc
Biotech Support Group LLC
1 Deer Park Drive, Suite M
Monmouth Junction NJ 08852
800-935-0628 North America