MONMOUTH JUNCTION, N.J. — Cleanascite ™ was used for lipid removal in an article published in the Journal of Thrombosis and Haemostaisis. For the localized control of acute thrombosis, scientists have developed a new platform that uses PPACK (Phe[D]-Pro-Arg-Chloromethylketone) with nanoparticles that serves as thrombin-inhibiting surfaces at sites of acutely forming thrombi to prevent the effects of local clot inhibition. A new article published on thrombin presents a thrombin inhibitor complexed with a nanoparticle as a anticoagulant with a multivalent thrombin adsorbing particle surface. In this article, PPACK and PPACK nanoparticle inhibition of thrombin were assessed in vitro via thrombin activity against a chromogenic substrate. Also in vivo acute arterial thrombosis model demonstrated that PPACK nanoparticles outperformed both heparin and uncomplexed PPACK in inhibiting thrombosis. So overall this article shows how Cleanascite ™ is used for sample preparation during experiments in vascular diseases to develop new products such as thrombin-inhibiting perfluorocarbon nanoparticles that may novel strategy for the treatment and magnetic resonance imaging of acute thrombosis.

The article mentions how Cleanascite™ is used: “The extent of PPACK coupling was determined by reverse-phase HPLC quantification f uncoupled PPACK after centrifugation of nanoparticles with Cleanascite ™ lipid adsorption reagent from Biotech Support Group.”
Cleanascite ™ has numerous benefits for lipid removal:
• A high binding capacity for lipids with minimal cross-reactivity with proteins
• Effectively replaces chlorinated/fluorinated hydrocarbons (eg. freon) and it is environmentally friendly.
• Helps purify antibodies, recombinant proteins, nucleic acids, proteoglycans
• Ideal for clarifying ascites, serum, cell & tissue culture, bile and organ homogenates
• Clarifies saliva and fecal components
• Very low protein binding
• Does not bind to DNA, RNA, enzymes and proteins
• Leaves glycoproteins, antibodies, nucleic acids, hemoglobin, proteoglycans, nucleic acids, serum components(such as hormones, nutrients, globulins, clotting factors, transport proteins) alone
• Extends the life of membrane and chromatographic columns.
• Ideal for delipidation treatments for downstream processing of large-scale therapeutic proteins, enzymes and monclonal antibodies.

For more information on Cleanascite: http://www.biotechsupportgroup.com/product_info.php?products_id=34

MYERSON, J., HE, L., LANZA, G., TOLLEFSEN, D. and WICKLINE, S. (2011), Thrombin-inhibiting perfluorocarbon nanoparticles provide a novel strategy for the treatment and magnetic resonance imaging of acute thrombosis. Journal of Thrombosis and Haemostasis, 9: 1292–1300. doi: 10.1111/j.1538-7836.2011.04339.x

About Biotech Support Group LLC

Biotech Support Group LLC is a leading developer of proteomic and genomic sample preparation and enrichment products. Its principal products include: AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ & ProPrep™ for nucleic acid isolation. For more information, go to www.biotechsupportgroup.com.

CONTACTS:

Dr. Swapan Roy & Matthew Kuruc
Biotech Support Group LLC
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Monmouth Junction NJ 08852
732-274-2866 Worldwide
800-935-0628 North America
sales@biotechsupportgroup.com
http://www.biotechsupportgroup.com