NuGel™ Polymer Coated Silica Affinity Matrices For Affinity Purification of Proteins.
MONMOUTH JUNCTION, N.J. — Silica has been an industry standard as an advantageous matrix suitable for high performance liquid chromatography. Through a proprietary polymer coating, silica is cross linked forming a reactive poly-epoxy functionality stable across a wide pH range (pH 2 to 10). From this foundational chemistry, all of the NuGel™ affinity products are derived.

• NuGel™ Poly-Epoxy has terminal epoxy as matrix reactive group and amino group as the ligand reactive group with direct coupling of amino groups.
• NuGel™ Poly-Amine has terminal amine as matrix reactive group and carboxylic acid or carbohydrate group as the ligand reactive group with carbodiiamide reaction or NaIO4 derived aldehyde reaction.
• NuGel™ Poly-Aldehyde has terminal aldehyde as matrix reactive group and amino group as the ligand reactive group with direct coupling of amino groups.
• NuGel™ Poly-Hydroxy has terminal glycol as matrix reactive group and amino as the ligand reactive group with carbodiiamidazole mediated reaction.
• NuGel™ Poly -Carboxy has terminal carboxylic acid as matrix reactive group and amino group as the ligand reactive group with carbodiiamide mediated reaction.
• NuGel™ Poly -NHS has terminal N-Hydroxy succinimide as matrix reactive group and amino group as the ligand reactive group with direct coupling of amino groups.

NuGel™ polymer coated silica affinity matrices has applications for enzyme immobilization, separation and purification of biopolymers, immobilization of proteins & ligands – monoclonal antibodies, hormones, peptides, haptens, drugs, etc.

Applications of NuGel™ Affinity Matrices

Ehrlich, G. K., Michel, H., Chokshi, H. P. and Malick, A. W. Affinity purification and characterization of an anti-PEG IgM. Journal of Molecular Recognition, 22: 99–103 (2009).
Anti-PEG IgM Purification : Scientists used PEG-derivatized NuGel™ to bind anti-PEG IgM for purifying anti-PEG IgM. Subsequent analysis of anti-PEG IgM and fragments occurred using PEG functionalized proteins/peptides by surface plasmon resonance, western blotting and ELISA. Such studies create probes for assays used in functionalized PEG molecules.

Membrane-based receptor affinity chromatography. Journal of Chromatography A Volume 597, Issues 1-2, 24 April 1992, Pages 155-166 9th International Symposium on Affinity Chromatography and Biological Recognition

Receptor Affinity Chromatography: In Purification and Analysis of Recombinant Proteins authors Seetharam and Sharma cite NuGel™ affinity support immobilization which allows for efficient receptor coupling and recovery of binding capacities. Authors recommend NuGel™ for industrial scale operations because of mechanical rigidity and flow rates which provide a durable bed support resulting in four fold flux than compared to agarose supports. Authors present the use of receptor affinity chromatography of recombinant interleukin 2 for the purification of recombinant proteins.

Purification of immunoglobulins using affinity chromatography and peptide ligands. Ruben Carbonell et al US Patent 2006/0153834 A1
Affinity Purification Of Human Immunoglobulins Using Immobilized Peptide Ligands on NuGel™ Poly-Amine Support: NuGel™ Poly-Amine is used for the purification of human immunoglobulins (Igs), a class of plasma proteins produced by the immune system.

George K. Ehrlich, Pascal Bailon, Wolfgang Berthold. Phage Display Technology – Identification of Peptides as Model Ligands for Affinity Chromatography Affinity Chromatography Methods in Molecular Biology, 2000, Volume 147, 209-220
Screening of Phage Peptide Libraries: In the procedure for screening of phage peptide libraries against monoclonal antibodies, scientists used NuGel™ for coupling peptide mimotope to the affinity matrix for purifying antibodies from human serum. Producing peptides on bacteriophages requires selection for ligand-binding molecules ie selection against target molecules and tailoring ligands to numerous applications. Authors Ehrlich et al describe the selection of peptide ligands for affinity chromatography (especially peptides that mimic antigen epitopes).

About Biotech Support Group LLC

Biotech Support Group LLC is a leading developer of proteomic and genomic sample preparation and enrichment products. Its principal products include: AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ & ProPrep™ for nucleic acid isolation. For more information, go to www.biotechsupportgroup.com.

CONTACTS:

Dr. Swapan Roy & Matthew Kuruc
Biotech Support Group LLC
1 Deer Park Drive, Suite M
Monmouth Junction NJ 08852
732-274-2866 Worldwide
800-935-0628 North America
sales@biotechsupportgroup.com
http://www.biotechsupportgroup.com

References:
Patents
Monoclonal antibodies directed to the cytotoxic lymphocyte maturation factor European Patent EP0790255
Purification of immunoglobulins using affinity chromatography and peptide US 2006/0153834 A1
Affinity
Dermot Walls, Robert McGrath and Sinéad T.Loughran A Digest of Protein Purification. Methods Molecular Biology. Volume 681: 3-23 (2011)
Ehrlich, G. K., Michel, H., Chokshi, H. P. and Malick, A. W. Affinity purification and characterization of an anti-PEG IgM. Journal of Molecular Recognition, 22: 99–103 (2009).
Development of hepatitis B virus capsids into a whole-chain protein antigen display platform: New particulate Lyme disease vaccines. International Journal of Medical Microbiology Volume 298, Issues 1-2, 3 January (2008), Pages 135-142
A sensitive and high-throughput assay to detect low-abundance proteins in serum Hongtao Zhang, Xin Cheng, Mark Richter & Mark I Greene. Nature Medicine 12, 473 – 477 (2006)