MONMOUTH JUNCTION, NJ.– Providing unrivalled specificity, NuGel™ Glycoprotein Enrichment PBA Kit contains phenyl boronic acid which covalently reacts with 1,2-cis-diol group–containing molecules. This unique chemistry eliminates the requirement for forming complex saccharide recognition motifs for interaction. The PBA ligand binds to the 1,2-cis-diol groups of biomolecules and enriches for heterogeneous sets of glycoproteins containing both N-linked and O-linked oligosaccharides. The specialized heterogenous nature of NuGel™ Glycoprotein Enrichment PBA Kit’s organo-boronic acid groups makes it applicable to all kinds of glycopeptides regardless of the sizes, structures, and hydrophilicities. The boronic acids facilitate the capturing of heterogeneous groups of O-linked oligosaccharides.

NuGel™ Glycoprotein Enrichment PBA Kit provides good reproducibility, simple protocol, and high-throughput capability. For detailed structural characterization of low-abundance glycoproteins, NuGel™ Glycoprotein Enrichment PBA Kit enriches glycoproteins from blood, serum, plasma, tissue or cell culture media. Glycoprotein isolation and enrichment might lead to biomarkers eg: biomarkers may be glycoproteins present in only minute quantities in tissue extracts and physiological fluids.

Examples Of Hetergenous Glycoproteins Enriched From Four Different Mammalian Samples

• Cis-diol specific, enriches heterogeneous sets of glycoproteins
• Unmasks glycoproteins from high abundance proteins, most notably albumin
• Disposable, easy spin filter format, no cross-contamination
• Efficient new surface technology ideal for proteomic applications
• Enriches glycoproteins from blood, serum, plasma, tissue or cell culture media.
• Removes > 90% of serum albumin (the non-glycosylated fraction)
• Sorbitol elution at pH 7.5 – 8.5

About Biotech Support Group LLC

Biotech Support Group LLC is a leading developer of proteomic and genomic sample preparation and enrichment products. Its principal products include: AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ & ProPrep™ for nucleic acid isolation. For more information, go to www.biotechsupportgroup.com.

CONTACTS:

Dr. Swapan Roy & Matthew Kuruc
Biotech Support Group LLC
1 Deer Park Drive, Suite M
Monmouth Junction NJ 08852
732-274-2866 Worldwide
800-935-0628 North America
sales@biotechsupportgroup.com
http://www.biotechsupportgroup.com

Suggested References

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3. Dai Z, Liu YK, Cui JF, Shen HL, et al. Identification and analysis of altered alpha1,6-fucosylated glycoproteins associated with hepatocellular carcinoma metastasis. Proteomics. 2006;6:5857–67.
4. Zhao J, Simeone DM, Heidt D, Anderson MA, et al. Comparative serum glycoproteomics using lectin selected sialic acid glycoproteins with mass spectrometric analysis: application to pancreatic cancer serum. J Proteome Res. 2006;5:1792–802.
5. Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D. T., Loo J. A. (2006) Identification ofN-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry. J. Proteome Res. 5, 1493–1503
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7. Yamashita K, Koide N, Endo T, Iwaki Y, et al. Altered glycosylation of serum transferrin of patients with hepatocellular carcinoma. J Biol Chem. 1989;264:2415–23.