MONMOUTH JUNCTION, NJ.– Authors Pottiez et al published a chapter in the book Expression Profiling in Neuroscience, Neuromethods titled, Proteomic Profiling of Cerebrospinal Fluid, on proteomic profiling platforms which analyze cerebrospinal fluid (CSF) for protein biomarkers and developing protein profiles of CSF for early identification of neurological diseases. Authors provide examples of affinity-based systems for removing most abundant proteins and cite Albusorb™ albumin depletion kit from Biotech Support Group. Moreover variations of protein concentration yielded by immunodepletion of CSF samples from nondemented (ND) patients and patients with HIV-associated dementia (HAD) are recorded. For detailed protocols click
http://link.springer.com/protocol/10.1007%2F978-1-61779-448-3_13

Albusorb™ is an albumin depletion reagent supplied as a kit with necessary buffers. AlbuSorb™ comes from a silica-based, separation platform utilizing a new combination of surface microenvironments substituted with low molecular substrates that feature drug-binding motifs. Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and cross-contamination.

Characteristics of Albusorb™

• Removes >90% albumin from 30 mg albumin/ml sample
• Affinity-type equivalence, virtually no cross-reactivity with other proteins
• Bind and elute procedure – simply weigh powder, condition the sample, centrifuge and/or filter, and recover the albumin depleted serum
• Economical new surface technology, not based on affinity chromatography
• Mild conditions maintain tertiary structure of proteins and simple transfer to secondary analysis
• The albumin depleted filtrate retains the enzymatic and biological activity
• Removes albumin from samples such as serum, plasma and from species including human, mouse, sheep, bovine, goat, rat, and calf
• The flow through fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.For more information about AlbuSorb™, click:
  http://www.biotechsupportgroup.com/node/64Biotech Support Group also has AlbuVoid™ for albumin depletion plus low abundance serum protein enrichment.
  AlbuVoid™ – Albumin Depletion Kit from Serum or Plasma
  http://www.biotechsupportgroup.com/node/55
  
  About Biotech Support Group LLC
  Biotech Support Group LLC is a leading developer of proteomic and genomic sample preparation and enrichment products. Its principal products include: AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ & ProPrep™ for nucleic acid isolation. For more information, go to www.biotechsupportgroup.com.
  
  CONTACTS:
  Dr. Swapan Roy & Matthew Kuruc
  Biotech Support Group LLC
  1 Deer Park Drive, Suite M
  Monmouth Junction NJ 08852
  732-274-2866 Worldwide
  800-935-0628 North America
  sales@biotechsupportgroup.com
  http://www.biotechsupportgroup.com
  http://www.biotechsupportgroup.com
  
  AlbuSorb™ References

• Rat Serum

  Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.
  http://www.pnas.org/content/early/2011/06/08/1019553108.abstract?elq=06e…

  Cerebrospinal Fluid

  Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270
  http://www.springerlink.com/content/j4u06x2k73u11476/#section=1013451&pa…

  Serum

  Happonen KE, Fürst CM, Saxne T et al. PRELP protein inhibits the formation of the complement membrane attack complex.Journal of Biological Chemistry.2012;287(11):8092-100
  http://www.ncbi.nlm.nih.gov/pubmed/22267731

  Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.
  http://www.pnas.org/content/early/2011/06/08/1019553108.abstract?elq=06e…

  Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats Nutrition and Cancer.2011;63(2):227-33
  http://www.researchgate.net/publication/49814854_Effects_of_Nobiletin_on…

  Holmberg, Rebecka Apolipoprotein CIII and Ljungan virus in diabetes 2010. Doctoral Thesis
  http://publications.ki.se/jspui/handle/10616/39507

  Lu Q, Zheng X, McIntosh T Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Analytical Chemistry.2009;81(21):8715-23
  http://www.ncbi.nlm.nih.gov/pubmed/19788250

  Suggested References

  Kabat, Elvin A., Murray Glusman, and Vesta Knaub. “Quantitative estimation of the albumin and gamma globulin in normal and pathologic cerebrospinal fluid by immunochemical methods.” The American journal of medicine 4.5 (1948): 653-662.
  http://www.sciencedirect.com/science/article/pii/S0002934…

  Cutler, R. W. P., G. V. Watters, and J. P. Hammerstad. “The origin and turnover rates of cerebrospinal fluid albumin and gamma-globulin in man.”Journal of the neurological sciences 10.3 (1970): 259-268.
  http://www.sciencedirect.com/science/article/pii/0022510X…

  Ganrot, Karin, and Carl-Bertil Laurell. “Measurement of IgG and albumin content of cerebrospinal fluid, and its interpretation.” Clinical chemistry 20.5 (1974): 571-573.
  http://www.clinchem.org/content/20/5/571.short

  Habgood, M. D., et al. “A developmentally regulated blood-cerebrospinal fluid transfer mechanism for albumin in immature rats.” The Journal of physiology456.1 (1992): 181-192.
  http://jp.physoc.org/content/456/1/181.short

  Müller, Norbert, and Manfred Ackenheil. “Immunoglobulin and albumin content of cerebrospinal fluid in schizophrenic patients: relationship to negative symptomatology.” Schizophrenia research 14.3 (1995): 223-228.
  http://www.sciencedirect.com/science/article/pii/0...

  Stevens, R. W., et al. “Application of fluoroimmunoassay to cerebrospinal fluid immunoglobulin G and albumin.” Journal of Clinical Microbiology 10.3 (1979): 346-350.
  http://jcm.asm.org/content/10/3/346.short

  Cutler, Robert WP, Ruthmary K. Deuel, and Charles F. Barlow. “Albumin exchange between plasma and cerebrospinal fluid.” Archives of Neurology 17.3 (1967): 261.
  http://archneur.ama-assn.org/cgi/reprint/17/3/261.pdf