|
Urinary proteomics sample preparation of proteinuria in exosomes by albumin depletion using AlbuSorb™ Nov. 18, 2013 – MONMOUTH JUNCTION, N.J. — Urine exosome isolation via ultracentrifugation followed by albumin depletion provides researchers with knowledge of renal regulation and could lead to the identification of biomarkers for diabetic nephropathy and diabetic mellitus. By removing albumin using AlbuSorb™ authors identified more urinary proteins such as flotillin-2, lamp-1, PODXL, tsg-101 from exosome fractions of urine samples. Authors Zubiri et al published an article in the Journal of Proteomics which cites AlbuSorb™ for high abundance protein depletion. Titled, ‘Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis’, the article cites protocols of proteomic workflows for biomarker discovery involving isolating exosomes from urine. Proteinuria in urine samples causes contamination of urine samples for proteomics research. Sample preparation protocols with depletion could complement precipitation techniques as less contamination is present in exosomal fractions upon depletion, enrichment, concentration and sample clarification. Urine exosome isolation via ultracentrifugation upon performing depletion provides researchers with knowledge of renal regulation and could lead to the identification of biomarkers for diabetic nephropathy and diabetic mellitus. Upon depletion of high abundance proteins such as albumin, urine exosomes from diabetic and health controls were analyzed by LC-MS/MS and selected reaction monitoring (SRM). The research cites AMBP, MLL3, VDAC1 as proteins in urinary exosomes of diabetic nephropathy patients. AlbuSorb™ is an albumin depletion reagent supplied as a kit with necessary buffers. AlbuSorb™ binds to albumin, and serum proteins flow through. Mild conditions maintain tertiary structure of proteins and simple transfer to secondary analysis. The flow through fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.
Journal of Proteomics detailed protocol Characteristics of AlbuSorb™
For more information visit: AlbuSorb ™ Albumin depletion kit visit
CONTACT:
References: Zubiri, Irene, et al. Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis. Journal of Proteomics (2013). *For research use only |
- About
- Products
- Hemoglobin Removal Kits
- Lipid Removal & Clarification
- Urine Protein & Low Abundance Enrichment
- Class Specific Enrichment
- Sample Prep Mass Spectrometry
- Functional & Chemical Proteomics
- Genomic Sample Prep
- Accessories
- Technical Resources
- References
- Publications & Reports
- FAQs
- Case Studies
- NRicher™ Bead Platform Provides Unique Sub-Proteome Biases And Fit For Purpose Opportunities for Targeted LC-MS Quantification
- BSG Products To Assist in Analyzing Macrophage Polarization
- Ectodomain Shedding and Enrichment of the Soluble Membrane Proteome
- Investigate out of the Venn Diagram box
- Methods to selectively deplete or purify Hemoglobin from Dried Blood Spots (DBS)
- The Utility of HemoVoid™ is Demonstrated in 3 Proteomic Investigations Identifying Potential Disease Specific Biomarkers
- The 4 common features of our sample prep products, known as the BSG Advantage, are highlighted in a selection of journal references.
- AlbuVoid™ Workflows Advance Cell Secretome Proteomics
- Lipid Removal for Phenotypic Cell Response in Cancer Research
- The Influence of Sample Prep Bias on LC-MS Targeted Peptide Quantification in Serum Proteomics
- Re-imagining proteomics for developing precision medicine biomarkers of the innate immune response in SARS-CoV-2
- Patent Application Describes New Proteomic Methods to Monitor Protease Inhibitor Function During Covid-19 Infections
- Efficient Hemoglobin Removal Advances Red Cell Proteomics Offering Many New Insights Into Inflammation and Infectious Disease
- The Potential for New Blood Biomarkers in the Management of COVID-19 Disease
- Establishing the Utility of HemoVoid™ and HemogloBind™ as Enrichment Tools for Proteomic Analysis of Red Cells and Whole Blood in Parkinson’s Disease
- Species Diversity Supported By BSG Products
- Poster Report Describes Loss of Functional Serpin Activity In Cancer Patient Blood
- AlbuVoid™️ PLUS & AlbuSorb™️ PLUS Evaluating Different Windows of Observation Solves The Many Challenges of Serum Proteomics
- Tackling the Challenges of Serum Proteomics
- Lipid Removal Sample Prep for Cell Response Applications
- Sample Prep for Proteomic Analysis of Saliva
- Biotech Support Group Featured in Book, "Functional Proteomics – Methods and Protocols"
- Sample Prep Liquid Biopsy Products Suitable for Proteomic Profiling of a Variety of Body Fluid Sample Types
- Albumin and High Abundance Depletion
- Using HemogloBind™ as a Hemoglobin Binding Reagent
- Diverse technologies available for researchers to selectively bind or enrich exosomes and extracellular vesicles.
- Stroma Liquid Biopsy™ Biomarkers Profile Pan-Cancer Dysregulation of the Serum Proteome
- Diverse Depletion and Enrichment Technologies Enhance Simplicity and Efficiency of Obtaining Quality Proteomic Information
- Use On-Bead Digestion to Improve Time Required for Serum Digestion
- Using AlbuVoid™ as a Serum Protein Enrichment Kit in Functional Proteomics
- Using Cleanascite™ as a Lipid Absorption and Clarification Reagent
- Using HemoVoid to Remove Hemoglobin Before Analysis
- Blog
- Contact
- Liquid Biopsy