Sample preparation of bile and serum samples containing mucin5AC glycoprotein using Cleanascite™ for cholangiocarcinoma & biliary tract tumor research

MONMOUTH JUNCTION, NJ — Cholangiocarcinoma (CCA) is the malignant spread of biliary tree epithelial cells. Growth of cholangiocytes could be caused by primary sclerosing cholangitis, liver fluke clonorchis sinensis, hepatolithiasis, hepatitis C virus infection etc. Lipid clarification is essential on liver bile samples containing lipids, cholesterol, phospholipids. Proper sample preparation using Biotech Support Group’s Cleanascite™ could identify biomarkers of cancer in bile and serum containing samples. Authors Danese et al describe the role of mucin5AC on bile samples of cancerous and non-cancerous patients. Biliary tract tumors, cholangiocarcinoma tissues, bile and serum samples express mucin 5AC (MUC5AC) glycoprotein. An enzyme-linked immunosorbent assay was performed to obtain MUC5AC quantification from bile and serum samples of extrahepatic cholangiocarcinoma and benign biliary diseases. MUC5AC expression as a serum/bile ratio was used to differentiate cholangiocarcinoma from cholangitis, cholangiocarcinoma from biliary stones and cholangitis from biliary stones. The article quotes “Delipidation was performed as follows: After centrifugation, the supernatant of each sample was mixed with 250 μL of Cleanascite (v/v ratio Cleanascite per sample = 1:4) and kept under mild agitation at 4°C for 1 hour to increase the agglomeration of fine lipids.”

The citation is:
Danese, Elisa, et al. “Assessment of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic performance and biologic significance.“ Surgery (2014).

Characteristics of Cleanascite™

• Ideal for clarifying ascites, serum, cell & tissue culture, bile and organ homogenates
• Clarifies saliva and fecal components
• Very low protein binding
• Does not bind to DNA, RNA, enzymes and proteins
• Leaves glycoproteins, antibodies, nucleic acids, hemoglobin, proteoglycans, nucleic acids, serum components(such as hormones, nutrients, globulins, clotting factors, transport proteins) alone
• Extends the life of membrane and chromatographic columns.
• Enrichment of delipidated tissue samples
• Ideal for delipidation treatments for downstream processing of large-scale therapeutic proteins, enzymes and monoclonal antibodies.

For more information visit:

Cleanascite™ – Lipid Removal Reagent and Clarification
http://www.biotechsupportgroup.com/node/73

About Biotech Support Group LLC

Biotech Support Group LLC is a leading developer of proteomic and genomic sample preparation and enrichment products. It’s principal products include: AlbuVoid™ & AlbuSorb™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, HemogloBind™ & HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™ & ProPrep™ for nucleic acid isolation. For more information, go to http://www.biotechsupportgroup.com

Contact Biotech Support Group:
Dr.Swapan Roy
Biotech Support Group LLC
1 Deer Park Drive, Suite M
Monmouth Junction NJ 08852
732-274-2866 Worldwide
800-935-0628 North America
sales@biotechsupportgroup.com
http://www.biotechsupportgroup.com

Suggested References
Hofmann, A. F. “Bile acid secretion, bile flow and biliary lipid secretion in humans.” Hepatology (Baltimore, Md.) 12.3 Pt 2 (1990): 17S-22S.

Ruzzenente, Andrea, et al. “A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC).” Surgery 155.4 (2014): 633-639.