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Patent Application Cites AlbuVoid™ For Secretome Sample Preparation & Neuroscience Research MONMOUTH JUNCTION, NJ — Biotech Support Group reports on a recent patent application which supports the utility of their proteomic sample preparation technology on albumin depletion for neuroscience research. The patent application is: Narain, Niven Rajin, and Paula Patricia Narain. “COMPOSITIONS AND METHODS FOR DIAGNOSIS AND TREATMENT OF PERVASIVE DEVELOPMENTAL DISORDER.” U.S. Patent No. 20,150,023,949. 22 Jan. 2015. The patent provides albumin sample preparation research application to track modulators of disease processes and experiment data on metabolomics, transcriptomics, single nucleotide polymorphisms using sample preparation and an artificial intelligence based informatics platform. Serum containing media are reduced using columns, reconstituted with buffers and serum albumin is depleted using Biotech Support Group’s AlbuVoid™. Culture medium optimization and subsequent modifications to buffer exchange is performed. Protein extraction requires a lysis buffer with protease inhibitors, vortexing, ultrasonication, centrifugation and clean up of debris. Secretome sample preparation of cultures and conditioned media requires reduction using dithiothreitol (DTT), alkylation using iodoacetamide and desalting using acetone precipitation. Quantitation of proteins using Bradford assay is performed and reduction, alkylation, digestion using trypsin is performed. Pooled aliquots of tryptic digests are labeled using iTRAQ, and analyzed using liquid chromatography mass spectrometry.
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Patent – Secretome Sample Preparation Narain, Niven Rajin, Rangaprasad Sarangarajan, and Vivek K. Vishnudas. “INTERROGATORY CELL-BASED ASSAYS AND USES THEREOF.” U.S. Patent No. 20,120,258,874. 11 Oct. 2012. Suggested References Kumar, Amit, et al. “Coupling enrichment methods with proteomics for understanding and treating disease.” PROTEOMICS-Clinical Applications (2015). Liu, Guowen, et al. “A Novel and Cost Effective Method of Removing Excess Albumin from Plasma/Serum Samples and Its Impacts on LC-MS/MS Bioanalysis of Therapeutic Proteins.” Analytical chemistry 86.16 (2014): 8336-8343.
Posted on Date:
Mon, 02/09/2015
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