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Albumin & IgG Removal Kits


AlbuVoid™

Selectively Voids Albumin, Binds Low Abundance Proteome

  • Albumin voids in flow through, >95%
  • <30 minute protocol
  • Low abundance enrichment equivalent or better than hexapeptides or antibodies
  • On-bead digestion protocols, efficient LC-MS workflows
  • Disposable, cost-effective, no column regeneration or cross-contamination
  • Mild elution maintains native structure with retained enzymatic, functional & bioactivities
  • Species agnostic
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2DE analysis of AlbuVoid™ treated sheep serum

2DE analysis of AlbuVoid™ treated sheep serum. Samples were reduced, alkylated and total protein normalized. The circled regions indicate the albumin zone. 1: Control. 2: AlbuVoid™ Eluate. Bottom: AlbuVoid™ Eluate silver stain.

AlbuSorb™

Selectively Binds Albumin

  • Removes serum albumin >90%
  • Economical small ligand surface architecture (not dye-based), bio-affinity performance
  • Consumable, cost-effective, no column regeneration or cross-contamination
  • Species agnostic
  • Compatible with:
    LC-MS
    Microarrays
    Functional assays
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Non-reduced SDSPAGE profiles

Non-reduced SDSPAGE profiles

Left lane: Normal human pooled serum control

Right lane: Flow-through from AlbuSorb™

AlbuVoid™ LC-MS On-Bead

Selectively Voids Albumin, Binds Low Abundance Proteome before LC-MS use

  • Seamless workflows
  • Label, label free & glyco- compatible
  • Unique proteolytic efficiencies
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Total LC-MS/MS Proteins identified at 2 digestion times


Total LC-MS/MS Proteins identified at 2 digestion times: 4 hours (4h) & overnight (O/N), single 3 hour LC gradient.

AlbuVoid™ PLUS

Albumin and IgG Depletion From Serum/Plasma for Proteomics

  • IgG removal >90% (70-80% of total Immunoglobulins removed)
  • Albumin removal >95%
  • Seamless and simple < 1 hour protocol
  • Low abundance enrichment equivalent to immuno-affinity
  • Disposable, cost-effective, no column regeneration or cross-contamination
  • Works for most species tested including human, sheep, rat, mouse, bovine
  • On-bead protocols improve workflow and efficiency, especially suited to targeted proteomics
  • Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional Assays.
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SDS-PAGE: Comparison of three methods using Human Serum Sample


20µg of protein loaded on to 10% tris bis gel

A - Serum alone – shows IgG and Albumin regions
B - NuGel™ ProteinA treated serum – shows IgG depletion
C - AlbuVoid™ treated serum – shows Albumin depletion
D - AlbuVoid™ PLUS treated serum – shows IgG and Albumin depletion

AlbuSorb™ PLUS

Selectively Binds Albumin & Immunoglobulin

  • 400 μg total serum protein mass per prep
  • > 85% Albumin, 85% IgG depleted from 25 μl serum
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AlbuSorb™ combines with an optimized immobilized Protein A to create AlbuSorb™ PLUS

AlbuSorb™ combines with an optimized immobilized Protein A to create AlbuSorb™ PLUS. Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and crosscontamination.

Lane 1: Human Serum Control

Lane 2: Serum after treatment with AlbuSorb™ PLUS


AlbuSorb™ LS

Suspension Reagent for Albumin Depletion From Serum or Plasma

  • Removes 30 mg albumin/ml, >90%
  • Affinity-type equivalence, virtually no cross-reactivity with other proteins
  • Disposable, no column regeneration or cross-contamination
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AlbuTrial™ Kit

Don’t know which one to try? Try both. The AlbuTrial kit has 1 gram of AlbuSorb™, and 5 preps of AlbuVoid™

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AlbuTrial™ PLUS Kit

Don’t know which one to try? Try both. The AlbuTrial PLUS kit has 5 preps of AlbuSorb™ PLUS, and 5 preps of AlbuVoid™ PLUS

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NuGel™ Protein A Kit

IgG Depletion From Serum/Plasma for Proteomics

  • IgG removal >90% (70-80% of total Immunoglobulins removed) from serum or cell culture
  • Supplied as dry stable bead format
  • Seamless and simple <1 hour protocol
  • Disposable, cost-effective, no column regeneration or cross-contamination
  • Works for most species tested including human, sheep, rat, mouse, bovine
  • Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional Assays
  • IgG recoverable with acidic eluent (optional)
  • Compatible with automation/high-throughput DPX Technologies XTR tip format
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Our BSG Advantage


Consumable

Cost-effective, not derived from biologicals

Cost-effective, not derived from biologicals

  • No specialized instruments or HPLC
  • Economical surface chemistries, not derived from biologicals
  • No regeneration, so no prep to prep variability
  • Simple, fast microfuge bind/wash/elute protocols

On-Bead Digestion

Efficient workflows, quality LC-MS/MS data

Efficient workflows, quality LC-MS/MS data

  • Simple, reproducible workflows
  • Equivalent or better than in-solution digestion
  • Seamless to LC-MS, no desalting or C18 separations
  • Unique proteolytic efficiencies

Enrichment/Depletion

Diverse strategies, species agnostic

Diverse strategies, species agnostic

  • Products support strategies for both enrichment of low abundance proteomes, or depletion of high abundance proteins
  • Species agnostic not derived from biologicals

Functional Integrity

Maintained throughout all separations

Maintained throughout all separation

  • Mild buffer conditions maintains native structure with retained enzymatic, functional & bio-activities
  • Supports enzyme biomarker assays
  • Functional & Chemical Proteomics
  • Structural & activity-probe Proteomics
  • Top-down & ArrayBridge PEP Proteomics
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