AlbuSorb™ PLUS - Albumin + IgG Depletion Kit From Serum or Plasma

Unmasks lower-abundance proteins
Species Agnostic
Suitable for LCMS, ELISA, Western Blot, Functional Analysis
Retains function and bio-activity

Product Code: APK285

>400 µg total serum protein mass (> 85% Albumin, >85% IgG depleted) from 25 µl serum prep
Affinity-type equivalence, virtually no cross-reactivity with other proteins
Disposable, no column regeneration or cross-contamination
Combines unique bead technology, not based on Blue-dye affinity, with optimized Protein A
Mild conditions maintains structural integrity and simple transfer to secondary analysis
Suitable for immunoassay, Western blot, 1 & 2D Electrophoresis, enzyme assay, LC-MS
Tested species include human, sheep, bovine, rabbit, mouse, rat
Validated in the automation compatible high-throughput DPX Technologies XTR tip format

Poly-electrolytesare polymers with repeating units of stationary charges. AlbuSorb™ comes from a class of solid-phase, or surface-based, elastomericpoly-electrolytic surfaces that bind proteins through an empiricallyderived chemistry combining elements of polymer composition,cross-linking architecture and charge properties. AlbuSorb™combines with an optimized immobilized Protein A to create AlbuSorb™PLUS .

Unlikeimmuno-affinity, the surfaces utilized are disposable eliminatingcycle to cycle variance and cross-contamination. AlbuSorb™PLUS is supplied as a powder. Simply weigh, centrifuge and/or filter, andrecover the {albumin + Immunoglobulin} - depleted serum in thesupernatant.

Click here to view AlbuSorb™ PLUS Product Sheet

Product	Size	# Serum Preps	Item No.
AlbuSorb™ PLUS	20 preps	20, 25 µl Serum Samples	APK285-20
AlbuSorb™ PLUS	100 preps	100, 25 µl Serum Samples	APK285-100

Kit includes:

Items	Item No APK285-20	Item No APK285-100	Reagent
AlbuSorb™ PLUS	1 Gram 20 Preps	5 Grams 100 Preps	Supplied
Binding Buffer BB1	30 ml	150ml	Supplied
Spin-X Filters	20	100	Supplied

References for AlbuSorb™ PLUS

Serum/Plasma

Sovakova,Magdalena, et al. "Colchicineacutely reduces liver-secreted inflammatory and metabolic proteins inblood."European Journal ofPharmacology (2026):178725. After AlbuSorb™ PLUS depletion of Albumin &Immunoglobulins, quantitative Data Independent Acquisition (DIA) massspectrometry uncovered modulation of the serum and plasma proteome ofliver-derived components, leading to systemic metabolic and immunechanges.

Serum
FraserDD, Roy S, Kuruc M, Quintero M, Van Nynatten LR, Cepinskas G, ZhengH, Soherwardy A and Roy D (2025) Functionalmass spectrometry indicates anti-protease and complement activityincrease with COVID-19 severity . Exp.Biol. Med. 250:10308.doi: 10.3389/ebm.2025.10308

Plasmasamples
Grantz,Jillian M., et al. "Theplatelet and plasma proteome and targeted lipidome in postpartumdairy cows with elevated systemic inflammation ." ScientificReports 14.1(2024): 31240.

Whole blood, serum and plasma samples
DPX Technologies and Biotech Support Group Announce In-Market Partnership for High Throughput Proteomic Sample Prep

Serum/Plasma Samples
Biotech Support Group & Lawson Health Research Institute Enter Collaborative Research Agreement to Monitor Protease Inhibitor Function During Covid-19 Infections

Serum
Thangavelu, Bharani, et al. " Dataset of Rat and Human Serum Proteomes Derived from Differential Depletion Strategies prior to Mass Spectrometry. " Data in Brief (2020): 105657.

Application Report

AlbuVoid™ PLUS & AlbuSorb™ PLUS - Evaluating Different Windows of Observation Solves The Many Challenges of Serum Proteomics

AlbuSorb™ Product Extension combines Albumin and Immunoglobulin Depletion in a Consumable Format

References For AlbuSorb™
Cerebrospinal Fluid
Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270
Synovial fluid
Happonen KE, Fürst CM, Saxne T et al. PRELP protein inhibits the formation of the complement membrane attack complex.Journal of Biological Chemistry.2012;287(11):8092-100
Serum
Zheng H, Soherwardy A, Roy S, Kuruc M, Avadhani S. AlbuVoid™ Enrichment & Antibody Depletion – Tackling the Challenges of Serum Proteomics Part II. Poster reprint first presented at 26th International Molecular Med TRI-CON, March 10-15, 2019, San Francisco, CA USA

Roy, Swapan, and Matthew Kuruc. "Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors." Functional Proteomics. Humana Press, New York, NY, 2019. 41-54.

Dr.Swapan Roy; Amenah Soherwardy; Ravish Amin; Matthew Kuruc Biotech Support Group LLC, Monmouth Junction, NJ - Application Report. AlbuSorb™ Product Extension combines Albumin and Immunoglobulin Depletion in a Consumable Format

Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.
Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats Nutrition and Cancer.2011;63(2):227-33
Holmberg, Rebecka Apolipoprotein CIII and Ljungan virus in diabetes 2010. Doctoral Thesis
Lu Q, Zheng X, McIntosh T Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Analytical Chemistry.2009;81(21):8715-23
Urine
Zubiri, Irene, et al. "Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis." Journal of proteomics 96 (2014): 92-102.
Patent
Berggren, Per Olaf, Yang, Shao-Nian. 2012. Methods For Treating And/Or Limiting Development Of Diabetes.U.S. Patent 20120328630 Kind Code: A1, filed June 25, 2012, and issued December 27, 2012.

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Click Here To View AlbuSorb™ PLUS Safety Data Sheet - AlbuSorb PLUS

Click Here To View AlbuSorb™ PLUS Safety Data Sheet - BB1

Product Code	Name	Base Price
Product Code:APK285-20	AlbuSorb™ PLUS - 20 preps	Base Price:$740.00
Product Code:APK285-100	AlbuSorb™ PLUS - 100 preps	Base Price:$3,090.00

AlbuSorb™ PLUS - Albumin + IgG Depletion Kit From Serum or Plasma

Description

Kit includes:

References

FAQ