Albumin and IgG Depletion From Serum/Plasma for Proteomics

IgG removal >90% (70-80% of total Immunoglobulins removed)
Albumin removal >95%
Seamless and simple < 1 hour protocol
Low abundance enrichment equivalent to immuno-affinity
Disposable, cost-effective, no column regeneration or cross-contamination
Works for most species tested including human, sheep, rat, mouse, bovine
On-bead protocols improve workflow and efficiency, especially suited to targeted proteomics
Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional Assays.

The classical plasma proteins generally fall into functional categories, forming the vast majority of the mid-to-high abundance proteome. In serum, these sub-proteomes by mass content are: Albumin 50-60%; Immunoglobulins 10-20%; Transport Proteins (Transferrin, Apo) 5-10%; Complement related Proteins 3-5%; Protease Inhibitors 2-5%; and all others 2-5%. While these sub-proteomes are required for normal body homeostasis, they nevertheless can become dysfunctional during acute-phase and chronic stimuli.

So, depending on the needs of the investigation, it can be valuable to consider that one or more of these categorical sub-proteomes is simply background noise whereby depletion is beneficial. While in other cases, these same categorical sub-proteomes might provide new data and information and consequently, should not be depleted. Different AlbuVoid™, AlbuVoid™ PLUS and AlbuSorb™ PLUS workflows support different proteomic biases as outlined in the following Table.

Click Here For The AlbuVoid™ Plus Product Sheet.

Products and digest conditions produce different sub-proteome windows of observation. So, depending on the needs of the investigation, it can be valuable to consider that one or more of these categorical sub-proteomes is simply background noise whereby depletion is beneficial. While in other cases, these same categorical sub-proteomes might provide new data and information and consequently, should not be depleted. Categorically the acute-phase sub-proteomes differentiated in disease may vary greatly from those associated with chronic sub-proteomes. So there is great benefit in having options to enrich or deplete one or more of these sub-proteomes.

BSG's Albumin and IgG Removal Kits offer many such options:

The 'PLUS' products substantially deplete Immunoglobulins through separations at the protein level.
The variable regions of Immunoglobulins are extremely heterogeneous, generating a background noise across the full LC gradient. On-bead digestion (BASP™) with AlbuVoid™ substantially reduces the influence of such Ig peptide features. So in addition to workflow simplicity, BASP™ can be advantageous utilized in targeted proteomic workflows whenever the target proteins do not require strong denaturing conditions.
With the exception of Immunoglobulins whereby FASP generates many more spectral features, both strong denaturing conditions (FASP) and on-bead digest (BASP™) conditions produce similar protein profiles.
Both Apolipoproteins and heavily glycosylated proteins (i.e., α1-Acid Glycoprotein) bind poorly to AlbuVoid™. For quantitative studies within these classes of proteins, AlbuSorb™ PLUS is recommended.
The Complement sub-proteome is especially enriched by AlbuVoid™ PLUS. The digest conditions may bias towards one or more functional sub-populations, likely due to conformational transitions and protein-protein interactions (i.e., Factor Bb, Properdin) that occur upon activation. This needs further investigation.
The low abundance sub-proteome is enriched 5+ fold with AlbuVoid™ and 4+ fold with AlbuSorb™ PLUS.

Product	Size	# Serum Preps	Item No.
AlbuVoid™ PLUS Kit	10 preps	10, 25µl Serum samples	NP-AVK-10

Items	Item No. NP-AVK-05	Item No. NP-AVK-10	Reagents
Kit#1 - IgG Depletion Kit
NuGel™ Protein A Beads (NP)	300mg	600mg	Supplied
Buffer 1	5 ml	10 ml	Supplied
Spin-X Centrifuge tube Filters	5	10	Supplied

Items	Item No. NP-AVK-05	Item No. NP-AVK-10	Reagents
Kit#2 - Albumin Depletion Kit
AlbuVoidTM Beads (AVK)	125mg	250mg	Supplied
Binding Buffer AVBB pH 6.0	3 ml	6 ml	Supplied
Wash Buffer AVWB pH 7.0	12 ml	24 ml	Supplied
Elution Buffer AVEB pH 10	2 ml	4 ml	Supplied
Spin-X Centrifuge tube Filters	5	10	Supplied
DTT, Iodoacetamide, Trypsin and Formic Acid	Not Supplied

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AlbuVoid™ PLUS - Albumin and IgG Depletion From Serum/Plasma for Proteomics

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