HemogloBind™ Kit

Product Code: H0155

HemoglobinRemoval and Capture

Has a high degree of specificity for hemoglobin, without cross-reacting with other proteins or analytes
Suitable for
- Hemolyzed serum/plasma
- Whole Blood, Dried Blood Cards (DBS) or Erythrocyte lysates
- Tissue Homogenates, compatible with RIPA buffer
Applications in analytical interferences, enzyme monitoring, proteomics
Species Agnostic
Over 30 citations including analysis in cellular thermal shift assay (CETSA), LC-MS proteomics, Hemoglobin derivatives, Western blot, enzyme activity, ELISA
Related product NuGel™HemogloBind™ comes in a dry powder format, compatible with high throughput 96-well automation, see https://www.biotechsupportgroup.com/NuGel-HemogloBind-Hemoglobin-Capture-Reagent-p/np-ho.htm

Poly-electrolytesare polymers with repeating units of stationary charges. HemogloBind™ is derived from insoluble elastomericpoly-electrolytes that bind proteins through an empirically derivedchemistry combining elements of polymer composition, andcross-linking architecture. As with bio-polymers like DNA andHeparin, governing their reactivity is the spatial presentation ofelectrostatic groups along a flexible polymer chain.

HemogloBind™does not cross react with most common serum components, making it anexcellent tool in numerous applications. These include analyticalprotocols where optical interference is problematic. Hemoglobinvariants, as in thalassemia, bind with differential affinity towardsHemogloBind™, though this has not been fully evaluated. Forpurification and/or analysis of hemoglobin, a modest elevation in pHwill facilitate desorption from the polymer.

Click Here To View HemogloBind™ Product Sheet
Click Here For The HemogloBind™ Hemoglobin Depletion Performance On Blood Sheet
Click Here For The HemogloBind™ Hemoglobin Depletion Performance On Dried Blood Spots/Cards

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Product Code	Name	Base Price	Qty
Product Code:H0155-20S	HemogloBind™ Kit - 20 preps hemolyzed serum, includes 2 ml HemogloBind™, 20 spin-filter assemblies	Base Price:$540.00