"The spike control sample showed a recovery value of 100.5 +/- 2.7%. The average recovery rate obtained after eNOS supplementation for the three brain homogenate specimens (rats #1, 2, and 3) was 18.7%. The treatment to remove lipds from a rat brain tissue homogenate sample with Cleanascite increased the mean recovery rate to 37.3 +/- 0.3% (n=4)."

"The low recovery values found with the crude brain tissue homogenates implies the presence of a large proportional matrix effect. we attempted to reduce this effect... The first approach was cleanascite treatment of brain tissue homogenate samples. The limited increase (from 27-37%) of the rate of recovery obtained after using this lipid-removing reagent suggests that: 1) lipids, which comprise approximately 12% of wet brain tissue mass [34], only contribute a portion of the negative interference observed from brain tisssue homogenates... it did not alter the estimate of the eNOS concentration, indicating that it did not remove the eNOS protein from within the sample..."

The quotes above are from the article, "Variations of brain endothelial nitric oxide synthase concentration in rat and mouse cortex," by Kenneth Czambel, Alexander Kharlamov, and Stephen C. Jones.