Viraffinity - Virus and Viral Component Isolation

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Viraffinity™ - Virus and Viral Component Isolation

Product Code: V1062

Purifies whole infectious non-enveloped virus & non-infectious enveloped virus
Prepares viral samples for subsequent detection and analysis
Alternative or complementary to ultracentrifugation
- “…love this vs sucrose cushions!” Maggie L. Bartlett, BS PhD, Johns Hopkins Bloomberg School of Public Health
- or can complement ultracentrifugation, for viral proteomic enrichment 350X ( Fragnoud et al, 2016 http://www.ncbi.nlm.nih.gov/pubmed/26572220)
Isolates antigenic virions, enveloped and non-enveloped
Enriches for viral nucleic acids; detergent, chaotrope, Trizol® compatible

Viraffinity™ is a unique water-insoluble elastomeric polyelectrolyte that has been engineered for the capture and recovery of viruses. It is supplied as a suspension reagent ready for use. Applications include: purification of whole infectious non-enveloped virus, virions, viral components, and sample preparation for subsequent detection and analysis.

Viraffinity™ is directly added to a pre-conditioned sample, is then mixed and centrifuged using standard micro-centrifuges (not ultra). The centrifuged pellet contains polyelectrolyte-bound viruses that can then be recovered using a moderately alkaline pH solution.

Click Here For Viraffinity™ Product Sheet

Related Viraffinity™ Kits

ViraPrep™ Mammal contains Viraffinity™ and all necessary buffers and protocols for mammalian virus and virion isolation.

https://www.biotechsupportgroup.com/ViraPrep-Mammal-p/vpm.htm

ViraPrep™Lambda is a complete application kit containing Viraffinity™ and all the necessary reagents for obtaining high purity DNA from plate or liquid lysates, suitable for amplification, automated fluorescent sequencing and other common molecular biology techniques.

https://www.biotechsupportgroup.com/ViraPrep-Lambda-p/vlk.htm

Product	size	Item No.
Viraffinity™	15ml	V1062-15

Cell Culture Supernatant Fluids
Yeh, Jane X., et al. "Treatment of Sindbis Virus-Infected Neurons with Antibody to E2 Alters Synthesis of Complete and nsP1-Expressing Defective Viral RNAs ." Mbio (2022): e02221-22.
Since the COVID-19 virus can be detected in stool samples, it has been proposed that detection of the virus in wastewaters will help to monitor local or regional outbreaks. For this to succeed, simple and robust measurement of viruses from wastewater is necessary. Conventionally, enteric viruses are first concentrated by a variety of filter methods. A secondary enrichment step is often employed, to reduce to a final volume and improve purity prior to final analysis. This secondary step may be provided by the Viraffinity™ reagent, and does not require ultracentrifugation. As described in these two references: 1) Viraffinity™ can dramatically improve the purification of viruses, and 2) can remove RT-PCR inhibitors in the detection of enteric viruses.
1) Romain Fragnoud R., et al. "Differential proteomic analysis of virus enriched fractions obtained from plasma pools of patients with dengue fever or severe dengue". BMC Infectious Diseases (2015) 15:518. http://www.ncbi.nlm.nih.gov/pubmed/26572220
The article's authors report a method to compare the proteomes of virion-enriched fractions purified from plasma pools of patients with dengue fever or severe dengue. Virions were purified by ultracentrifugation combined with Viraffinity™. “…and became more intense after the Viraffinity™ step...Densitometry indicated that the protein complexity of the purified samples was reduced by roughly 350-fold compared to the unpurified samples.”.
2) Leggitt, Paris R., and Lee-ann Jaykus. "Detection methods for human enteric viruses in representative foods." Journal of Food Protection 63.12 (2000): 1738-1744.
“To optimize viral nucleic acid amplification, secondary PEG precipitates… required a prior adsorption step with an equal volume of Viraffinity™ to further remove RT-PCR inhibitors. In this case, viruses …were adsorbed by the addition of an equal volume of Viraffinity™, … These Viraffinity™ precipitates were used directly in subsequent RNA extractions.”

Patents
Bedin, Frédéric, and Romain Fragnoud. "Method and kit for determining the probability that a patient will develop a severe case of dengue." U.S. Patent Application No. 15/912,730.

Parthasarathy, Ranjani V., Hsi-Chou Liu, Wensheng Xia, Assumpta Bennaars-Eiden, Paul N. Holt, William Bedingham, Christopher R. Kokaisel, and Jeffrey C. Pederson. " Methods for nucleic acid amplification ." U.S. Patent Application 12/596,346, filed April 25, 2008.

Parthasarathy, Ranjani, Katya Ericson, and William Bedingham. " Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent ." U.S. Patent Application 10/852,022, filed May 24, 2004.
Bedingham, William, et al. " Variable valve apparatus and methods ." U.S. Patent No. 8,057,758. 15 Nov. 2011.
Parthasarathy, Ranjani, Katya Ericson, and William Bedingham. " Methods for nucleic acid isolation and kits using solid phase material ." U.S. Patent Application No. 10/852,645.

Parthasarathy, Ranjani V., Katya Ericson, and William Bedingham. " Methods for nucleic acid isolation and kits using a microfluidic device and concentration step. " U.S. Patent 7,939,249, issued May 10, 2011.

Bedingham, William, Barry Robole, Ranjani Parthasarathy, and Katya Ericson. "Variable Valve Apparatus and Methods." U.S. Patent Application 11/684,656, filed March 12, 2007.

Bedingham, William, et al. " Variable Valve Apparatus and Methods. " U.S. Patent Application No. 11/684,656.
European Patent Application EP2305809 Methods for nucleic acid isolation and kits using a microfluidic device and concentration step

Journal Citations
Dengue Virus
Fragnoud, Romain, et al. " Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with dengue fever or severe dengue ." BMC infectious diseases 15.1 (2015): 518.

Food Samples
Maurer, John, ed. PCR methods in foods . Springer Science & Business Media, 2006.

D’Souza, D. H. " Update on foodborne viruses: types, concentration and sampling methods. " Advances in Microbial Food Safety 2 (2014): 102.
Jaykus, Lee-Ann. " Detection of human enteric viruses in foods. " Foodborne Diseases Handbook 2 (2000): 137-163.
Sair, A. I., D. H. D'souza, and L. A. Jaykus. " Human enteric viruses as causes of foodborne disease ." Comprehensive reviews in food science and food safety 1.2 (2002): 73-89.
Lee, A. " Rapid Detection of Foodborne Viruses from Minimally Processed Foods , poster Australian Society of Microbiology." (2000).

Grohmann, Gary, and Alvin Lee. " Viruses, food and environment." Foodborne microorganisms of public health significance . Sydney: AIFST (1997): 603-620.
Savers, Screen. " EXHIBIT DESCRIPTIONS. " Microbial & Comparative Genomics 2.3 (1997): 244-249.
Use of Viraffinity and Multiplex RT-PCR for the dectection of Food borne viruses
Food and Water Samples
Grohmann, Gary, and Alvin Lee. " Viruses, food and environment." Foodborne microorganisms of public health significance. Sydney: AIFST (1997): 603-620.
Isometric Virus
Zeddam, Jean-Louis, et al. " An isometric virus of the potato tuber moth Tecia solanivora(Povolny)(Lepidoptera: Gelechiidae) has a tri-segmented RNAgenome ." Journal of invertebrate pathology 99.2 (2008): 204-211.
Hantaan Virus
Yu, Hai-Tao, Hong Jiang, Ye Zhang, Xue-Ping Nan, Yu Li, Wei Wang, Wei Jiang et al. " Hantaan virus triggers TLR4-dependent innate immune responses ."Viral immunology 25, no. 5 (2012): 387-393.
Wang, Ping-Zhong, Chang-Xing Huang, Ye Zhang, Zhi-Dong Li, Hai-Tao Yu, Ying Zhang, Zhan-Sheng Jia et al. " Analysis of the immune response to Hantaan virus nucleocapsid protein C-terminal-specific CD8+ T cells in patients with Hemorrhagic fever with renal syndrome ." Viral immunology 22, no. 4 (2009): 253-260.

Jiang, Hong, Ping-Zhong Wang, Ye Zhang, Zhe Xu, Li Sun, Li-Mei Wang, Chang-Xing Huang et al. " Hantaan virus induces toll-like receptor 4 expression, leading to enhanced production of beta interferon, interleukin-6 and tumor necrosis factor-alpha. " Virology 380, no. 1 (2008): 52-59.

Human Enteric Viruses
Leggitt, Paris R., and Lee-ann Jaykus. " Detection methods for human enteric viruses in representative foods ." Journal of Food Protection® 63.12 (2000): 1738-1744.

Jaykus, Lee-Ann. " Detectionof human enteric viruses in foods. " Foodborne Diseases Handbook 2 (2000): 137-163.
Human Mouth Virus
Al-Jarbou, Ahmed. Metagenomic Analysis of the Human Mouth Virus Population and Characterisation of Two Lytic Viruses . Diss. University of Leicester, 2009.
Lepidoptera: Gelechiidae
Zeddam, Jean-Louis, et al. " An isometric virus of the potato tuber moth Tecia solanivora (Povolny)(Lepidoptera: Gelechiidae) has a tri-segmented RNA genome ." Journal of invertebrate pathology 99.2 (2008): 204-211.
Serum
Moreau, Isabelle, John Levis, Orla Crosbie, Elizabeth Kenny-Walsh, and Liam J. Fanning et al. " Correlation between pre-treatment quasispecies complexity and treatment outcome in chronic HCV genotype 3a ." Virology journal 5, no. 1 (2008): 1.

Abdurahman, Samir. Studies on HIV-1 core assembly . Institutionen för laboratoriemedicin/Department of Laboratory Medicine, 2007.
Human Immunodeficiency Virus Type 1
Abdurahman, Samir, Akos Végvári, Masoud Youssefi, Michael Levi, Stefan Höglund, Elin Andersson, Peter Horal, Bo Svennerholm, Jan Balzarini, Anders Vahlne, et al. " Activity of the small modified amino acid α-hydroxy glycineamide on in vitro and in vivo human immunodeficiency virus type 1 capsid assembly and infectivity ." Antimicrobial agents and chemotherapy 52, no. 10 (2008): 3737-3744.

Abdurahman, S., Höglund, S., Höglund, A., & Vahlne, A et al,. (2007). Mutation in the loop C-terminal to the cyclophilin A binding site of HIV-1 capsid protein disrupts proper virus assembly and infectivity . Retrovirology, 4(1), 1.
Abdurahman, Samir, Masoud Youssefi, Stefan Höglund, and Anders Vahlne, et al. " Characterization of the invariable residue 51 mutations of human immunodeficiency virus type 1 capsid protein on in vitro CA assembly and infectivity ." Retrovirology 4, no. 1 (2007): 1.

Abdurahman, Samir, Stefan Höglund, Laura Goobar-Larsson, and Anders Vahlne . " Selected amino acid substitutions in the C-terminal region of human immunodeficiency virus type 1 capsid protein affect virus assembly and release ." Journal of general virology 85, no. 10 (2004): 2903-2913.
Plasma
Fragnoud, Romain, Marie Flamand, Frederic Reynier, Philippe Buchy, Vasna Duong, Alexandre Pachot, Glaucia Paranhos-Baccala, and Frederic Bedin. " Differential proteomic analysis of virus-enriched fractions obtained from plasma pools of patients with dengue fever or severe dengue ." BMC infectious diseases 15, no. 1 (2015): 518.
Polio Virus
Ting, W.T. E ., E. M. Nielson, and C.C. Tseng. 1997. The use of Viraffinity matrix to concentrate waterborne polioviruses for RT-PCR detection . abstr. Q-169. p.484. In Abstracts of the 97th General Meeting of the American Society for Microbiology 1997, American Society for Microbiology, Washington D.C.

Bacteriophage Lambda DNA
Hitti, J. "Fast and Convenient Purification of Bacteriophage Lambda DNA with Viraffinity™ Matrix, poster Cold Spring Harbor Conference on Genome Mapping & Sequencing." (1997).

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Performance:

Virus	Titer	Ratio	% Bound b
HIV-1 ^a	7x10 ³ TCID₅₀	1:2	96
HIV-1, human serum	7x10 ³ TCID₅₀	1:2	80
Chimeric Human Rhinovirus ^a	10 ⁶ - 10⁸ pfu/ml	1:3	95
Adenovirus (Ad5d1309) ^a	10 ⁶ - 10⁸ pfu/ml	1:3	90
Reovirus Type 3 ^a	10 ⁶ - 10⁸ pfu/ml	1:4	99
Encephalomyocarditis (EMC) ^a	10 ⁷TCID₅₀	1:4	99
Porcine Parvovirus ^a	10 ⁷TCID₅₀	1:2	90
Unclassified Entero-Picornavirus ^a	10 ⁶TCID₅₀	1:4	90
Coxsackievirus A24 ^a	10 ⁶ - 10⁷ pfu/ml	1:2	70-95
Bacteriophage Lambda	10 ⁹ pfu/ml	1:5	>95

Ratio refers to the volumetric ratio of Viraffinity™ to sample.

^a Tissue culture supernatants containing 1-10% Fetal Bovine Serum.

^b Based on infectivity.

MSDS:
MSDS-Viraffinity™, Catalog Order # V0162

Product Code	Name	Base Price	Qty
Product Code:V1062-15	Viraffinity - 15 ml suspension reagent	Base Price:$635.00

Viraffinity™ - Virus and Viral Component Isolation

Description

Related Viraffinity™ Kits

References

FAQ