NuGel™ NRicher™ Mx

Product Code: SRPRO

Enrich low abundance proteomes

Enriches and normalized low abundance sub-proteomes 5-10X
Compress proteome concentrations
Tissue and species agnostic
Suitable for small volume mg scale preps
Composite NRicher™ 6 surfaces can be deconstructed and optimized for biomarker enrichment
Supports Compound Centric Displacement Proteomics (CCDP) method

BSG has developed a new method for proteome separationsbased on chemically derived weak affinity or imperfect fit interactions.Without the use of antibodies, progressive displacement allows the beads tobias for or against certain proteins, without compromising protein integrity.

The NuGel™-based NRicher™ products are adaptable to anymeasurable protein function from any sample type, to aid in the discovery ofnew biomarkers. They support all functional,activity-probe, chemical and top-down proteomic applications .

Click To Download NuGel™ NRicher™ Mx Low Abundance Enrichment
Click To Download NuGel™ NRicher™ Mx Compound Centric Displacement Proteomics

Suggested References

"NRicher™: A Singular Low Abundance Proteome Enrichment Product Line With Seamless Integration of On-Bead Digestion " Biotech Support Group to exhibit at the 71st ASMS Conference on Mass Spectrometry and Allied Topics, June 4 - 8, 2023 in Houston, Texas

The Functional & Chemical Proteomics Handbook 03/2016

Oka, Amita R., Matthew P. Kuruc, Ketan M. Gujarathi, and Swapan Roy. " Functional Proteomic Profiling of Phosphodiesterases Using SeraFILE Separations Platform." International Journal of Proteomics 2012 (2012).

New Chemical Proteomic Methods To Access Drug-Protein Interactions

US HUPO 2014. Frontiers in Proteomics: Advancing Biology through Technology and Computation.

NuGel™ PROfessor™ abstract entitled "Compound-Centric Displacement Proteomics - An Advantaged Method To Survey Small Molecule-Protein Interactions" poster board 096 presented at US HUPO 2014

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Kit includes:

Kit Contains:	NuGel™ NRicher™ Mx 10	NuGel™ NRicher™ Mx 50
NuGel™ NRicher™ Mx beads	150 mg	750 mg
PRO-CBB Binding Buffer, pH 6.0	3 ml	15 ml
PRO-CWB Wash Buffer pH 7.0	8 ml	40 ml
PRO-CEB Elution Buffer, pH 7.0	1 ml	5 ml
Spin-X microfuge filters	10	50

MSDS:

MSDS - NuGel™ NRicher™ Mx, Catalog Order # SRPRO-05, SRPRO-10, SRPRO-50

Product Code	Name	Base Price
Product Code:SRPRO-10	NuGel NRicher Mx	Base Price:$595.00
Product Code:SRPRO-50	NuGel NRicher Mx	Base Price:$2,785.00

NuGel™ NRicher™ Mx

Description

Enrich low abundance proteomes

References

FAQ