SurfactAway™ SDS Removal

Product Code: SA645

SurfactAway™ SDS Removal

Removes >99% detergent such as Triton, NP-40, Tween-20, CTAB, CHAPS, Lubrol and deoxycholate from protein solutions
Very selective, virtually no cross-reactivity with other proteins
Simple, just pipette, centrifuge and discard pellet
Economical new surface technology, not based on hydrophobic chromatography

Click Here To View SurfactAway™ Product Sheet

Detergents can often interfere with protein analysis. SurfactAway™ offers a simple and fast method to remove SDS and non-ionic detergents such as Triton. Recovery of protein is quantitative. SurfactAway™ SDS is especially designed for SDS removal and contains a conditioning buffer and a solid-phase binding suspension. A two-step, 10 minute process in a microfuge is all that's required. Surfactaway™ Non-ionic, is a solid-phase suspension reagent, just add and centrifuge.

Product	Size	# of Samples & sample Size*	Item No.
SurfactAway™ SDS	30 ml	120, 1 ml samples	SA645-30
SurfactAway™ SDS	250 ml	1000, 1 ml samples	SA645-250

*Based on a 1:4 SurfactAway to sample typical volume ratio.

Extraction and identification of electroimmunoprecipitated proteins from agarose gels. Journal of Immunological Methods Volume 330, Issues 1-2, 31 January 2008, Pages 24-33

Reyes, Levy A. New mechanisms in nitric oxide synthase related endothelial dysfunction in the isolated heart. Diss. The Ohio State University, 2012.

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Performance:

ANALYSIS	SPECIFICATION
APPEARANCE:	White gel suspension
GEL TO LIQUID RATIO :(10 minutes at 2000 x g)	1:4
pH :	7.0-8.0
PERFORMANCE SPECIFICATIONS: The test criteria below is used for quality assurance purposes only and challenges the product under extreme conditions. This should not be construed as an indication of efficacy for any specific application. Please consult the Application and Protocols document supplied with the product or Biotech Support Group’s Technical Service department for specific use guidelines and characteristics.
PROTEIN RECOVERY SPECIFICATIONS:
Sheep Serum (50 mg/ml), 0.1%SDS, reacted at 1:4 v/v Surfactaway™	>95%
Sheep Serum (50 mg/ml), 1%SDS, reacted at 1:4 v/v Surfactaway™	>95%

MSDS:
MSDS-SurfactAway™ Non-ionic, Catalog Order # SA890

Product Code	Name	Base Price
Product Code:SA645-30	SurfactAway SDS Removal - 30 ml suspension reagent	Base Price:$530.00
Product Code:SA645-250	SurfactAway SDS Removal - 250 mL suspension reagent	Base Price:$1,900.00

SurfactAway™ SDS Removal

Description

References

FAQ