NuGel™ Protein A Kit

Product Code: NP

NuGel™ Protein A Kit
IgG Depletion From Serum/Plasma for Proteomics

IgG removal >90% (70-80% of total Immunoglobulins removed) from serum or cell culture
Supplied as dry stable bead format
Seamless and simple <1 hour protocol
Disposable, cost-effective, no column regeneration or cross-contamination
Works for most species tested including human, sheep, rat, mouse, bovine
Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional Assays
IgG recoverable with acidic eluent (optional)
Compatible with automation/high-throughput DPX Technologies XTR tip format

Click here to view the NuGel™ Protein A Kit one-sheet.

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Click Here To View NuGel™ Protein A Kit Safety Data Sheet - NuGelProtein A
Click Here To View NuGel™ Protein A Kit Safety Data Sheet - BB1

Product Code	Name	Base Price
Product Code:NP-20	NuGel™ Protein A Kit - NP-20, 20 preps	Base Price:$525.00
Product Code:NP-100	NuGel™ Protein A Kit - NP-100, 100 preps	Base Price:$2,210.00

NuGel™ Protein A Kit

Description

FAQ