AlbuVoid™ PLUS - Albumin and IgG Depletion From Serum/Plasma for Proteomics

Unmasks lower-abundance proteins
Species Agnostic
Suitable for LCMS, ELISA, Western Blot, Functional Analysis
Retains function and bio-activity

Product Code: NP-AVK

Albumin and IgG Depletion From Serum/Plasma for Proteomics

IgG removal >90% (70-80% of total Immunoglobulins removed)
Albumin removal >95%
Seamless and simple < 1 hour protocol
Low abundance enrichment equivalent to immuno-affinity
Disposable, cost-effective, no column regeneration or cross-contamination
Works for most species tested including human, sheep, rat, mouse, bovine
On-bead protocols improve workflow and efficiency, especially suited to targeted proteomics
Suitable for LC-MS, 1 and 2D Gels, ELISAs, Enzyme and other Functional Assays.

The classical plasma proteins generally fall into functional categories, forming the vast majority of the mid-to-high abundance proteome. In serum, these sub-proteomes by mass content are: Albumin 50-60%; Immunoglobulins 10-20%; Transport Proteins (Transferrin, Apo) 5-10%; Complement related Proteins 3-5%; Protease Inhibitors 2-5%; and all others 2-5%. While these sub-proteomes are required for normal body homeostasis, they nevertheless can become dysfunctional during acute-phase and chronic stimuli.

So, depending on the needs of the investigation, it can be valuable to consider that one or more of these categorical sub-proteomes is simply background noise whereby depletion is beneficial. While in other cases, these same categorical sub-proteomes might provide new data and information and consequently, should not be depleted. Different AlbuVoid™, AlbuVoid™ PLUS and AlbuSorb™ PLUS workflows support different proteomic biases as outlined in the following Table.

Click Here For The AlbuVoid™ Plus Product Sheet.

Products and digest conditions produce different sub-proteome windows of observation. So, depending on the needs of the investigation, it can be valuable to consider that one or more of these categorical sub-proteomes is simply background noise whereby depletion is beneficial. While in other cases, these same categorical sub-proteomes might provide new data and information and consequently, should not be depleted. Categorically the acute-phase sub-proteomes differentiated in disease may vary greatly from those associated with chronic sub-proteomes. So there is great benefit in having options to enrich or deplete one or more of these sub-proteomes.

BSG's Albumin and IgG Removal Kits offer many such options:

The 'PLUS' products substantially deplete Immunoglobulins through separations at the protein level.
The variable regions of Immunoglobulins are extremely heterogeneous, generating a background noise across the full LC gradient. On-bead digestion (BASP™) with AlbuVoid™ substantially reduces the influence of such Ig peptide features. So in addition to workflow simplicity, BASP™ can be advantageous utilized in targeted proteomic workflows whenever the target proteins do not require strong denaturing conditions.
With the exception of Immunoglobulins whereby FASP generates many more spectral features, both strong denaturing conditions (FASP) and on-bead digest (BASP™) conditions produce similar protein profiles.
Both Apolipoproteins and heavily glycosylated proteins (i.e., α1-Acid Glycoprotein) bind poorly to AlbuVoid™. For quantitative studies within these classes of proteins, AlbuSorb™ PLUS is recommended.
The Complement sub-proteome is especially enriched by AlbuVoid™ PLUS. The digest conditions may bias towards one or more functional sub-populations, likely due to conformational transitions and protein-protein interactions (i.e., Factor Bb, Properdin) that occur upon activation. This needs further investigation.
The low abundance sub-proteome is enriched 5+ fold with AlbuVoid™ and 4+ fold with AlbuSorb™ PLUS.

Product	Size	# Serum Preps	Item No.
AlbuVoid™ PLUS Kit	10 preps	10, 25µl Serum samples	NP-AVK-10

Items	Item No. NP-AVK-05	Item No. NP-AVK-10	Reagents
Kit#1 - IgG Depletion Kit
NuGel™ Protein A Beads (NP)	300mg	600mg	Supplied
Buffer 1	5 ml	10 ml	Supplied
Spin-X Centrifuge tube Filters	5	10	Supplied

Items	Item No. NP-AVK-05	Item No. NP-AVK-10	Reagents
Kit#2 - Albumin Depletion Kit
AlbuVoidTM Beads (AVK)	125mg	250mg	Supplied
Binding Buffer AVBB pH 6.0	3 ml	6 ml	Supplied
Wash Buffer AVWB pH 7.0	12 ml	24 ml	Supplied
Elution Buffer AVEB pH 10	2 ml	4 ml	Supplied
Spin-X Centrifuge tube Filters	5	10	Supplied
DTT, Iodoacetamide, Trypsin and Formic Acid	Not Supplied

AlbuVoid™ PLUS & AlbuSorb™ PLUS - Evaluating Different Windows of Observation Solves The Many Challenges of Serum Proteomics

Zheng H, Soherwardy A, Roy S, Kuruc M, Avadhani S. AlbuVoid™ Enrichment & Antibody Depletion – Tackling the Challenges of Serum Proteomics Part II. Poster reprint first presented at 26th International Molecular Med TRI-CON, March 10-15, 2019, San Francisco, CA USA
Vialaret, Jerome & Kadi, Sarah & Tiers, Laurent & O Flynn, Robin & Lehmann, Sylvain & Hirtz, Christophe. (2018). Albumin depletion of human serum to improve quantitative clinical proteomics. Current Topics in Peptide & Protein Research 19. 53-62.
Poillet-Perez, Laura, et al. " Autophagy maintains tumour growth through circulating arginine." Nature (2018): 1.
Roy, Swapan, and Matthew Kuruc. " Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors." Functional Proteomics. Humana Press, New York, NY, 2019. 41-54.
Mihara, Keisuke, et al. " Identification of Specific Protein Markers of Rheumatoid Arthritis in Synovial Fluid and Serum." Journal of Hard Tissue Biology 27.1 (2018): 55-58.
David L. Wang, Chuanguang Xiao, Guofeng Fu, Xing Wang and Liang Li. Identification of potential serum biomarkers for breast cancer using a functional proteomics technology. Biomarker Research (2017) 5:11. DOI 10.1186/s40364-017-0092-9
Zheng, H., et al. " AlbuVoid™ coupled to on-bead digestion-tackling the challenges of serum proteomics." J Proteom Bioinformatics 8.9 (2015): 225.
Zheng, H., et al. Poster reprint first presented at 14th Human Proteome Organization World Congress (HUPO 2015), held on September 27 – 30, 2015 entitled “ New Proteomic Workflows Combine Albumin Depletion and On Bead Digestion, for Quantitative Cancer Serum”.

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Click Here To View SDS-NuGel Protein A Safety Data Sheet - AlbuVoid PLUS

Click Here To View AlbuVoid™ PLUS Safety Data Sheet - AlbuVoid PLUS

Click Here To View AlbuVoid™ PLUS Safety Data Sheet - AVBB

Click Here To View AlbuVoid™ PLUS Safety Data Sheet - AVWB

Click Here To View AlbuVoid™ PLUS Safety Data Sheet - AVEB

Click Here To View AlbuVoid™ PLUS Safety Data Sheet - Buffer 1

Product Code	Name	Base Price	Qty
Product Code:NP-AVK-10	AlbuVoidâ„¢ PLUS - Albumin and IgG Depletion From Serum/Plasma for Proteomics -10 preps	Base Price:$820.00

AlbuVoid™ PLUS - Albumin and IgG Depletion From Serum/Plasma for Proteomics

Description

References

FAQ