Silica has been an industry standard as an advantageous matrix suitable for high performance liquid chromatography. With NuGel™, non-specific sites have been virtually eliminated making it an ideal support for affinity purification. Through a proprietary polymer coating, silica is crosslinked forming a reactive Poly-Epoxy functionality stable across a wide pH range (pH 2 to 10). From this foundational chemistry, all of the NuGel™ affinity products are derived.
The Phenyl Boronic Acid (PBA) ligand is immobilized through the NuGel™ poly-Epoxy linkage with attachment through the amino group. While various lectins bind to specific saccharide residues, the PBA ligand binds to the 1,2-cis-diol groups of biomolecules and enriches for heterogeneous sets of glycoproteins containing both N-linked and O-linked oligosaccharides. An easy and fast spin-filter format makes glycoprotein enrichment simple starting from 50µl serum, or 1-2 mg total protein.
| Product | Size | Total samples processed | Item No. |
|---|---|---|---|
| NuGel™ GlycoproteinEnrichment PBA Kit | 10 Preps | 10X 50 µl of Serum or Plasma(1-2mg total protein each prep) | NGPBA-10 |
| NuGel™ GlycoproteinEnrichment PBA Kit | 50 Preps | 50X 50 µl of Serum or Plasma(1-2mg total protein each prep) | NGPBA-50 |
| Items | 10 Prep | 50 Prep | Reagent |
|---|---|---|---|
| NuGel™ Glycoprotein Enrichment PBA Matrix, NGPBA | 0.5 gram | 2.5 grams | Supplied |
| Binding Buffer NGPBA-BB, PH 8.5 | 5 ml | 25 ml | Supplied |
| Wash Buffer NGPBA-WB, PH 8.5 | 15 ml | 75 ml | Supplied |
| Elution Buffer NGPBA-EB, PH 8.5 | 5 ml | 25 ml | Supplied |
| SpinX Centrifuge tube filters | 10 | 50 | Supplied |
Performance:
| Sample | % Glycoprotein Eluted with Sorbitol (relative to starting sample) |
|---|---|
| Mouse Plasma | 33 |
| Rat Serum | 44 |
| Sheep Serum | 18 |
| Bovine Serum | 40 |
| Bovine Brain Homogenate | 9 |
