HemogloBind™ Nucleic Acid

Product Code: HN250

Suspension Reagent to remove Hemoglobin interference for PCR

Suitable for infectious disease research or rigorous analysis for whole blood or heavily hemolyzed samples
Scaleable suspension reagent
Robust and simple, no phenol/chloroform or ammonium sulfate precipitation
High Yield – free and soluble DNA or RNA is highly recoverable
> 95% Hemoglobin and Protein removal from whole blood lysates & dried blood cards
Eliminates inhibitory effects of heme for PCR applications
Compatible with silica-type final isolation preps

Some infectious viruses are known or suspected to be associated with red cells, such as Zika and West Nile viruses. So to detect their presence, it is becoming increasingly apparent to use whole blood, rather than plasma alone, as a starting sample type. However, the presence of hemoglobin from whole blood lysates is challenging for PCR because of the large amount of hemoglobin present. HemogloBind™ Nucleic Acid is designed to efficiently remove hemoglobin while maintaining very high recovery of nucleic acids, both RNA and DNA.

The protocol is easily scaled up or down to accommodate different starting blood volumes and works with common lysis buffers. Simply adjust the reagent volumes 5:1 proportionally to the starting blood volume.

Click Here To View HemogloBind™ Nucleic Acid Product Sheet

Performance Efficiency	HemogloBind™ Nucleic AcidSuspension Reagent: SampleVolume Ratio	NucleicAcidRecovery
RNA from Yeast, Total OD A₂₆₀ = 15	5 : 1	>95%

Performance Efficiency	HemogloBind™ Nucleic AcidSuspension Reagent	Hemoglobin Removal	Protein Removal
50 µl whole blood (Lysed using 3 M GuSCN. Final volume 150 µl.)	250 µl	>95%	>95%

Product	Item No.
HemogloBind™ Nucleic Acid - 20 preps, 50 µl whole blood	HN250-20

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

Product Code	Name	Base Price	Qty
Product Code:HN250-20	HemogloBind Nucleic Acid - 20 preps	Base Price:$530.00

HemogloBind™ Nucleic Acid

Description