NuGel&#153 BindPro - Protein Removal & Enrichment of Metabolites/Analytes From Serum or Plasma

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NuGel™ BindPro™ - Protein Removal & Enrichment of Metabolites/Analytes From Serum or Plasma

Specialized product line for glycoprotein enrichment
Species Agnostic
Suitable for LCMS, ELISA, Western Blot, Functional Analysis
Retains function and bio-activity

Product Code: BPM55

Aqueous Protein Crash &Enrichment of Metabolites/Analytes From Serum or Plasma

Serum and plasma protein removal, >95%
Ammonium Acetate buffer system, simplifies analyte concentration
Aqueous Protein Crash, linearly scalable, unlike chemical precipitation or membrane filtration.
Fast process, less than 30 minutes from application to separation
Applicable for drug binding/screening, target analytes and metabolomics
Species agnostic, tested species include: human, mouse, sheep, bovine, goat, rat, calf
Dry powder format, compatible with high throughput systems such as 96 well plate.
NuGel™ BindPro™ supplied as a dry powder reagent; related product - BindPro™ supplied as a suspension reagent

Click Here To View NuGel™ BindPro™ Product Sheet

BindPro™ is an umbrellatrademark for polymeric reagents designed as alternatives to ultra-filtrationand solvent precipitation for applications that require protein removal and/orconcentration in a more versatile or scalable format. NuGel™ BindPro™ is engineered ontoa bead format, based on passivated porous silica ( NuGel™ platform) covalentlybound to elastomeric poly-electrolytes. NuGel™ BindPro™can be used in lieu of solvents for drug binding studies, fortargeted analytes, or for metabolomic investigations.

Product	Size	# of Samples &Sample Size*	Item No.
NuGel™BindPro™	15 Preps	15 preps, 20-30 µl of serum	BPM55-15
NuGel™BindPro™	50 Preps	50 preps, 20-30 µl of serum	BPM55-50

Kit includes:

Items Required	15 prep	50 Prep	Reagent
BindPro™ Metabolomics	0.75 grams	2.5 grams	Supplied
Binding Buffer BPMBB, PH 6.0	8.0 ml	25 ml	Supplied
Elution Buffer BPMEB	8.0ml	25ml	Supplied
SpinX Centrifuge tube filters	15	50	Supplied

Performance:

Protein Removal Per Prep	Removal
BSA, 1 to 2 mg	>95%
Plasma or Serum 1 to 2 mg	>95%

Lipoproteins

Turner, Joseph D., R. Stuart Langley, Kelly L. Johnston, Katrin Gentil, Louise Ford, Bo Wu, Maia Graham et al. "Wolbachia lipoprotein stimulates innate and adaptive immunity through Toll-like receptors 2 and 6 to induce disease manifestations of filariasis." Journal of Biological Chemistry 284, no. 33 (2009): 22364-22378.
Patent

Bhogal, John, Shridhara Alva Karinka, Timothy P. Henning, David Cunningham, Udo Hoss, Andrew H. Naegeli, and John Latour. "Methods of Collecting and Analyzing Samples." U.S. Patent 20,120,296,189, issued November 22, 2012.

Do BSG's products work for all species?

Yes, as our products are not derived from antibodies, they are species agnostic, and have applied to humans, mice, rats, guinea pigs, goats, cows, pigs, even frogs and fish.

Can you supply bulk material only (from the kit-based products) for large volume orders?

Yes, we can adjust all of our kit products, to supply only the beads. For large orders, whether kit or bulk material, we provide special discounted quotations. Please inquire.

Can the protocol be scaled to accommodate different amounts of either volume or mass?

Yes, we provide general guidelines for use, but the protocols can usually be adjusted to accommodate either larger or small starting samples, or larger or smaller starting concentrations. We recommend first contacting us for guidance in adjusting the protocol.

Can the products be adapted to high throughput formats and automation?

Although we do not have an off-the-shelf product for 96-well formats currently, we have ongoing collaborations that can provide such formats. Please inquire.

Are the products compatible with detergents?

This has not been fully investigated, but we do not recommend use of SDS. Low concentrations, 0.1 – 0.5% of non-ionic detergents have been used with some of our products, but to what extent they alter separation performance, is unknown. If non-ionic detergents are to be used, generally we recommend that they be of no greater than 0.1% before applying to the beads. So for tissue lysates for example at higher %, dilute the sample before application.

What is the stability and shelf life of the product?

The products have a shelf life typically 6 months to 1 year. We ship at room temperature, but recommend storage at 4°C upon receipt. Do not freeze.

Do the products contain any biologically derived material?

All of our products are derived chemically, not biologically, with one exception. The "PLUS" products contain recombinant Protein A.

How can I order?

We can take on-line cart orders, which accept North American credit cards, Visa & MasterCard. It does not however, accept other international credit cards. If you would like to order direct, contact us at [email protected]. In the US, distributors Fisher Scientific and VWR can be used to order all products, even those not listed on their sites, just provide them with our catalog #. A full list of international distributors is listed on How to Order | Biotech Support Group LLC.

MSDS:

MSDS-BindPro™, Catalog Order # BP355

SDS:
SDS-NuGel™ BindPro™ (Catalog Order # BPM55-15, BPM55-50)

Product Code	Name	Base Price
Product Code:BPM55-15	NuGel BindPro - 15 preps	Base Price:$630.00
Product Code:BPM55-50	NuGel BindPro - 50 preps	Base Price:$1,325.00

NuGel™ BindPro™ - Protein Removal & Enrichment of Metabolites/Analytes From Serum or Plasma

Description

References

FAQ