Unlike other solid-phases, Cleanascite™ does not have significant protein binding making its selectivity profile for lipids unique in the bio-research products industry. As a result, it is ideal to clear lipid associated matrix effects from human sera, bile, ascites, and other high lipid content sample types.
Effectively replaced chlorinated/fluorinated hydrocarbons (eg. freon)
Proven workflows for antibodies, proteins, nucleic acids, proteoglycans, serum analytes, and cell response
Not derived from immuno-affinity
A high binding capacity for lipids with minimal cross-reactivity with proteins and nucleic acids
References for clarifying ascites, serum, cell & tissue culture, bile and organ homogenates
The authors aimed at simultaneously measuring intact endogenous-insulin and derived C-peptide, to help predict development of diabetes mellitus, as well as in differential diagnosis in cases of hypoglycemia. Cleanascite™ is shown both to improve LC-MS measurements, and validated in accordance with CLIA '88 guidelines. { doi: 10.1016/j.cca.2016.01.019 }
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To evaluate immunogenic response to a vaccine candidate, it is necessary to measure the antibodies from sera; a sample with a diverse lipid profile. In this citation, Cleanascite™ was used in a toxin neutralizing assay to evaluate the influence of cholesterol dependency, on a candidate protein pneumococcal vaccine. { doi: 10.1016/j.vaccine.2012.11.005 }
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The authors report methods to overcome the biological variability of analyzing a high number of bile samples. They concluded that delipidation yielded a considerable number of complementary protein identifications and that Cleanascite™ treatment was indispensable for in-solution digestion methods. { http://dx.doi.org/10.1016/j.jprot.2016.11.021 }
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The researchers determined the role of complement on MAb-mediated protection for four mice Ig subclasses. After centrifugation of ascites fluid, Cleanascite™ protocol was implemented to remove lipids. { doi: 10.1128/IAI.70.5.2598-2604.2002 }
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The applications and references for the many diverse investigations using Cleanascite™ upstream of cell response measurements are described in a downloadable whitepaper. { Cleanascite Cell Response Reference Applications }
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Cleanascite™” helped this study show that the metabolite methylmalonic acid (MMA) complexed with lipidic structures, is a circulatory serum factor that contributes to pro-aggressive effects from an older population in cancer, The authors conclude that lipid-complexed MMA drives a more aggressive phenotype in cancer cells and as such, is a promising therapeutic target for advanced carcinomas.
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