HemoVoid to Remove Hemoglobin Before Analysis
of a commercial resin, HemoVoid, to investigate the efficiency of hemoglobin
depletion technology before analysis
BSG has developed a
chemical library of general non-specific adsorbents, or stated another way -
beads with weak affinity or imperfect fit interactions.
the use of antibodies, progressive displacement allows the beads to bias for or
against certain proteins, without compromising protein integrity. Each product
is empirically characterized to meet the needs of the application. In
HemoVoid™, is designed to remove hemoglobin
from erythrocyte lysate samples in a simple and efficient manner.
Used by researchers studying the cytoplasmic
protein content of red cells who need to remove hemoglobin, the most abundant
protein in red cells, HemoVoid™ is used to enrich the low abundance proteome
A recent research article describing the
simplicity and efficiency of HemoVoid™, to enrich the soluble cytoplasmic
proteins in order to assess the activity of sGC, PDE, and PKG in Hb-free
They aimed to establish a
procedure that allowed for fast and reliable preparation of hemoglobin-free
cell lysates from as little as 1–2 ml blood.
As one of the main advantages of HemoVoid™,
the maintenance of functional activity post-separations provided fast,
reliable, functional integrity of enriched sub-proteome.
Additional key advantages include:
Hemoglobin voids in flow-through
>98%, with <30 minute bind/wash/elute protocol
Sample types include red blood
cells, whole blood, and dried blood cards
Species agnostic, validated on
human, mouse, sheep, goat, bovine
Without a reliable resin like HemoVoid™, the
study would have been compromised by the analytical noise introduced due to the
presence of hemoglobin.
This article illustrates the importance of not
only removing the influence of hemoglobin in order to perform proteomic
analysis of red cells, but that the activities of the soluble RBC enzymes are
preserved and can be monitored for differential function in disease.