Viraffinity™ - Virus and Viral Component Isolation
Viraffinity™
- Purifies whole infectious non-enveloped virus & non-infectious enveloped virus
- Isolates antigenic virions, enveloped and non-enveloped
- Enriches for viral nucleic acids
- Prepares viral samples for subsequent detection and analysis
Viraffinity™ is a unique water-insoluble elastomeric polyelectrolyte that has been engineered for the capture and recovery of viruses. Applications include: purification of whole infectious non-enveloped virus, virions, viral components, and sample preparation for subsequent detection and analysis. Viraffinity™ is directly added to the sample that is then mixed and centrifuged. The centrifuged pellet contains polyelectrolyte-bound viruses that can then be recovered using a moderately alkaline pH solution. Viraffinity™ is supplied as a suspension reagent ready for use. Simply pipette the suspension into the sample at the appropriate ratio, typically 1 volume of Viraffinity™; to 4 volumes sample. Viraffinity™ is also supplied as the enabling component of the ViraPrep™ application kits.
Click here for Viraffinity™ Product Sheet
Patents
Composition and utility patents for Viraffinity™ and related technologies.
References
Norovirus
Shinohara, Michiyo, Kazue Uchida, Shin-ichi Shimada, Kyoko Tomioka, Noriko Suzuki, Toshitaka Minegishi, Sachie Kawahashi, Yuko Yoshikawa, and Norio Ohashi. Application of a simple method using minute particles of amorphous calcium phosphate for recovery of norovirus from cabbage, lettuce, and ham.Journal of Virological Methods (2012)
Hanta VirusHai-Tao Yu, Hong Jiang, Ye Zhang, Xue-Ping Nan, Yu Li, Wei Wang, Wei Jiang, Dong-Qiang Yang, Wen-Jing Su, Jiu-Ping Wang, Ping-Zhong Wang, and Xue-Fan Bai.Hantaan Virus Triggers TLR4-Dependent Innate Immune Responses. Viral Immunology.2012;
Mihalic, K. A., et al, Development of a Chemiluminescent Western Blot for Detecting Hantaan-Specific Antibodies, poster American Society of Tropical Medicine and Hygiene Meeting, October 1997.
Human Mouth Virus
Metagenomic Analysis of the Human Mouth Virus Population and Characterisation of Two Lytic Viruses, Al-Jarbou, Ahmed, Theses, Dept. of Infection. Immunity and Inflammation.2009
Lepidoptera: Gelechiidae
An isometric virus of the potato tuber moth Tecia solanivora (Povolny) (Lepidoptera: Gelechiidae) has a tri-segmented RNA genome Jean-Louis Zeddam et. al Journal of Invertebrate Pathology, Volume 99, Issue 2, October 2008, Pages 204-211
Human Immunodeficiency Virus Type 1
Activity of the Small Modified Amino Acid -Hydroxy Glycineamide on In Vitro and In Vivo Human Immunodeficiency Virus Type 1 Capsid Assembly and Infectivity Antimicrobial Agents and Chemotherapy, October 2008, p. 3737-3744, Vol. 52, No. 10 Samir Abdurahman
Mutation in the loop C-terminal to the cyclophilin A binding site of HIV-1 capsid protein disrupts proper virus assembly and infectivity Samir Abdurahman , Stefan Höglund , Anders Höglund and Anders Vahlne Retrovirology 2007, 4:19doi:10.1186/1742-4690-4-19
Poliovirus type 1 (PV1), Hepatitis A virus (HAV), Norwalk virus
Detection Methods for Human Enteric Viruses in Representative Foods. Leggitt, Paris R.1; Jaykus, Lee-Ann, Journal of Food Protection®, Volume 63, Number 12, December 2000 , pp. 1738-1744(7).
Polio Virus Type 1 and Calcivirus
Rapid Detection of Foodborne Viruses from Minimally Processed Foods.Microbial Detection Methods 2000
Polio Virus
Ting, W.T. E., E. M. Nielson, and C.C. Tseng. 1997. The use of Viraffinity matrix to concentrate waterborne polioviruses for RT-PCR detection. Abstr. Q-169. p.484. InAbstracts of the 97th General Meeting of the American Society for Microbiology 1997, American Society for Microbiology, Washington, D.C.
Bacteriophage Lambda DNA
Hitti, J., et al, Fast and Convenient Purification of Bacteriophage Lambda DNA with Viraffinity Matrix, poster Cold Spring Harbor Conference on Genome Mapping & Sequencing, May 1997.
