PROspector - Differentiated Subproteomes and Enrich Functional Biomarkers

• Efficiently produce differentiated subproteomes with bioactivity preserved.
• Generate characteristic molecular profiles for comparison and discovery.
  
• Enrich functional biomarkers for sequence and structural annotation.
  
• Natural source variants compartmentalized for challenge/response with small molecules  

Efficiently produce differentiated subproteomes with bioactivity preserved
 The mild binding and elution conditions preserve the functional characteristics of the sub-proteomes, important for the next step  - the measurement of activity.  The SeraFILE-derived daughter sub-proteomes can be analyzed in many ways to generate a molecular profile. Unlike other proteomic platforms which rely exclusively on analyzing for protein content for example 2D electrophoresis and Mass Spec, ProFACTs molecular profiles can combine and correlate many aspects of functional, structural and expression measurements into one platform analyses.

 Generate characteristic molecular profiles for comparison and discovery
The ability to monitor protein function offers profiling capabilities that cannot be achieved with peptide mass fingerprinting. This is especially useful in profiling enzymes and their variants where the activity can measured under a variety of challenge conditions, including factors which activate the enzymes, factors especially small molecules, that inhibit the enzymes, and their substrates under different concentrations.

Enrich functional biomarkers for sequence and structural annotation
An enrichment strategy might be a select enzyme activity that you want to isolate, characterize and identify. You have pre-determined a way to monitor its activity but cannot observe the presence of the protein directly.   By comparing specific activities - enzyme activity divided by total protein, an enrichment factor can be calculated showing which sub-proteome had the highest enrichment for the selected activity. These select enriched sub-proteomes can be further subjected to analyses for sequence and structural annotation by common proteomic techniques, ie. MS. Optionally, these can fractionated further with the SeraFILE platform.
Natural source variants compartmentalized for challenge/response with small molecules   

PROspector allows one to catalog or index the effects of functional modulation. We think this will be especially valuable to those that want to establish panels of proteins that are responsive to small molecule drug modulation. For example, a client may have a small molecule kinase inhibitor, and wants to index which kinases, are functionally modulated. While this type of analysis is often done with panels of recombinantly produced enzymes, our methodology starts with natural cellular extracts much closer to physiological conditions. Therefore the protein content is functionally annotated in all its variant forms, mutations, splice variants regulatory factors, etc..
 

Click here to view PROspector™ Product Sheet