ProCipitate™ - Protein removal/DNA enrichment & isolation reagent, substitute to phenol/chloroform
- Non-hazardous substitute to phenol/chloroform
- Removes only the contaminants & leaves DNA alone
- Improves yield of DNA over alternative bind and elute systems
- Adaptable to any sample size, and can be automated
- Key component of the ProPrep™ line of application specific kits
ProCipitate™ is a unique protein removal reagent based upon patented elastomeric polyelectrolytes. The polymer chains of ProCipitate™ are initially extended in a high energy state due to an overall net negative charge. When introduced to protein solutions, the charges are neutralized and the polymer chains collapse to a more favorable energy state; DNA and RNA remain unreacted. ProCipitate™ is non-hazardous and can replace phenol/chloroform with the additional benefits of solid-phase suspensions: adaptability to filtration and automation. It is routinely used for plasmids, cosmids, BACs, and genomic DNA, as well as RNA. ProCipitate™ can also be used to remove Proteinase K and other enzymes. ProCipitate™ provides high quality DNA suitable for automated sequencing, southern blotting, and restriction digestion. ProCipitate™ is available as a suspension reagent and in ProPrep™ kits for specific applications and high-throughput 96 well filter formats.
For more information on ProCipitate™ P0050-50 Size & Quantity: 500ml, please contact us
Click here to view ProCipitate™ Product Sheet
ProCipitate™, Superior Substitute to Phenol/Chloroform for DNA Isolation & Protein Extraction. by Matt Kuruc February 23, 2015
U.S. Patent Numbers 5,294,681, 5,453,493 and other patents pending.
U.S. Patent Number 5,538,870, Method for Preparing Nucleic Acids For Analysis And Kits Useful Therefore.
Richards, Gary P., and Gail E. Greening. "Detection of enteric viruses in shellfish." Molluscan Shellfish Safety. Springer Netherlands, 2014. 177-183.
Schwab KJ; De Leon R; Sobsey MD. Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR. Applied and Environmental Microbiology.1996;62(6):2086-94
Schwab KJ, De Leon R, Sobsey MD Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR. Applied and Environmental Microbiology.1995; 61(2): 531-537
LA Jaykus, R De Leon and MD Sobsey. A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization Applied and Environmental Microbiology.1996; 62(6): 2074–2080
Mycobacterium Avium Paratuberculosis
Charles G. Thornton; Kerry M. MacLellan; Judith R. Stabel; Christine Carothers; Robert H. Whitlock; Selvin Passen Application of the C18-Carboxypropylbetaine Specimen Processing Method to Recovery of Mycobacterium avium subsp. paratuberculosis from Ruminant Tissue Specimens Journal of Clinical Microbiology.2002;40:5;1783-1790
Zoltan S.; Gyimesi; Ilse H.; Stalis; Janice M.; Miller; Charles O Detection of Mycobacterium avium Subspecies avium in Formalin-Fixed, Paraffin-Embedded Tissues of Captive Exotic Birds Using Polymerase Chain Reaction . Thoen Journal of Zoo and Wildlife Medicine.1999;30:3:348-353
Miller, J.M., et al, Polymerase chain reaction identification of Mycobacterium avium in formalin-fixed, paraffin-embedded animal tissues. Journal of veterinary diagnostic investigation.1999.11:436-440
Ellingson, J. L. E., et al. "Detection of Mycobacterium avium subspecies paratuberculosis in free-ranging bison (Bison bison) by PCR." Molecular and cellular probes 19.3 (2005): 219-225.
Recombinant Bacterial Colonies
O. Umesh K. Reddy; Alan E. Pepper; Ibrokhim Abdurakhmonov; Sukumar Saha; Johnie N. Jenkins; Thomas Brooks, Yuksel Bolek; Kamal M. El-Zik New Dinucleotide and Trinucleotide Microsatellite Marker Resources The Journal of Cotton Science.2001;5:103-113
Quiniou SM; Katagiri T; Miller NW; Wilson M; Wolters WR; Waldbieser GC Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus. Genetics, selection, evolution : GSE. 2003;35(6):673-83
Bacterial artificial chromosome (BAC) vector
J M Kelley; C E Field; M B Craven; D Bocskai; U J Kim; S D Rounsley; M D Adams. High Throughput Direct End Sequencing of BAC Clones. Nucleic Acids Research.1999.15;27(6):1539-1546
Carcinoma Cell Lines & Bacterial Artificial Chromosomes (BACs)
Alsop, Amber E., Andrew E. Teschendorff, and Paul AW Edwards. "Distribution of breakpoints on chromosome 18 in breast, colorectal, and pancreatic carcinoma cell lines." Cancer genetics and cytogenetics 164.2 (2006): 97-109.
Plasmid Deoxyribonucleic acid (DNA)
G.M. Huang; K. Wang; C.L. Kuo; B. Paeper; L. Hood A High-Throughput Plasmid DNA Preparation Method Analytical Biochemtry.1994;15;223(1):35-8
Robert R. Klein, Daryl T. Morishige, Patricia E. Klein, Jianmin Dong; John E. Mullet. High Throughput BAC DNA Isolation for Physical Map Construction of Sorghum. Plant Molecular Biology Reporter.1988;16(4):651-364
Deoxyribonucleic acid (DNA)
Kozekov ID; Turesky RJ; Alas GR; Harris CM; Harris TM; Rizzo CJ. Formation of Deoxyguanosine Cross-Links from Calf Thymus DNA Treated with Acrolein and 4-Hydroxy-2-nonenal. Chemical Research in Toxicology.2010;23(11):1701-1713
Applied Biosystems User Bulletin. Subject: Sequencing Large DNA Templates.
David C. Bruce; Mark O. Mundt; Kim K. McMurry; Linda J. Meincke; Donna L. Robinson; Norman A. Doggett; Larry L. Deaven. BAC Library End Sequencing in Support of Whole Genome Assemblies DOE Joint Genome Institute and Center for Human Genome Studies, Los Alamos National Laboratory
Dr. Domon, National Agricultural Research Center for Kyushu Okinawa Region, Japan, Extraction of Rush DNA, unpublished personal correspondence, 2004.
DNA Extraction Genetic Diversity as an Indicator of Ecosystem Condition and Sustainability for Regional Assessments of Stream Condition in the Eastern United States
Stephanie M Cohen, Terrence S Furey, Norman A Doggett, and David G Kaufman. Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts BMC Genomics.2006;7: 301
Roberto A. Rodríguez, Lauren Thie, Christopher D. Gibbons, Mark D. Sobsey. Reducing the effects of environmental inhibition in quantitative real-time PCR detection of adenovirus and norovirus in recreational seawaters. Journal of Virological Methods;2012:181(S1):43-50
Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR. Applied and environmental microbiology.1995;61(2):531-7
Krupey, J., et al, 100,000+ PCRs Possible from 10 ml Blood, poster Biotechniques Symposium, 2003.
Human T-cell lymphotrophic virus I-induced adult T-cell leukemia (ATL)
J D Burton; R N Bamford; C Peters; A J Grant; G Kurys; C K Goldman; J Brennan; E Roessler; T A Waldmann. A lymphokine, provisionally designated interleukin T and produced by a human adult T-cell leukemia line, stimulates T-cell proliferation and the induction of lymphokine-activated killer cells Proceedings of the National Academy of Sciences of the United States of America.1994. 91(11): 4935-4939
Zebrafish genomic library of bacterial artificial chromosome (BAC) clones and BAC DNA
McDermott, Brian M., et al. Transgenic labeling of hair cells in the zebrafish acousticolateralis system. Gene Expression Patterns 10.2 (2010): 113-118.