ProCipitate™
ProCipitate™- - Protein removal reagent/DNA enrichment & isolation, substitute to phenol/chloroform
- Non-hazardous substitute to phenol/chloroform
- Removes only the contaminants & leaves DNA alone
- Improves yield of DNA over alternative bind and elute systems
- Adaptable to any sample size, and can be automated
- Key component of the ProPrep™ line of application specific kits
ProCipitate™ is a unique protein removal reagent based upon patented elastomeric polyelectrolytes. The polymer chains of ProCipitate™ are initially extended in a high energy state due to an overall net negative charge. When introduced to protein solutions, the charges are neutralized and the polymer chains collapse to a more favorable energy state; DNA and RNA remain unreacted. ProCipitate™ is non-hazardous and can replace phenol/chloroform with the additional benefits of solid-phase suspensions: adaptability to filtration and automation. It is routinely used for Plasmids, Cosmids, BACs, and Genomic DNA, as well as RNA. ProCipitate™ can also be used to remove Proteinase K and other enzymes. ProCipitate™ provides high quality DNA suitable for automated sequencing, Southern blotting, and restriction digestion. ProCipitate™ is available as a suspension reagent and in ProPrep? kits for specific applications and high-throughput 96 well filter formats.
Click here to view ProCipitate™ Product Sheet
U.S. Patent Numbers 5,294,681, 5,453,493 and other patents pending.
U.S. Patent Number 5,538,870, Method for Preparing Nucleic Acids For Analysis And Kits Useful Therefore.
Transgenic labeling of hair cells in the zebrafish acousticolateralis system Brian M. McDermott Jr Gene Expression Patterns Volume 10, Issues 2-3, February-March 2010, Pages 113-118
Formation of Deoxyguanosine Cross-Links from Calf Thymus DNA Treated with Acrolein and 4-Hydroxy-2-nonenal. Ivan D. Kozekov, Robert J. Turesky, Guillermo R. Alas, Constance M. Harris, Thomas M. Harris, Carmelo J. Rizzo Chemical Research in Toxicology 2010 23 (11), 1701-1713
Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts Stephanie M Cohen, Terrence S Furey, Norman A Doggett, and David G Kaufman BMC Genomics. 2006; 7: 301. Published online 2006 November 29. doi: 10.1186/1471-2164-7-301.
Dr. Domon, National Agricultural Research Center for Kyushu Okinawa Region, Japan, Extraction of Rush DNA, unpublished personal correspondence, 2004.
Krupey, J., et al, 100,000+ PCRs Possible from 10 ml Blood, poster Biotechniques Symposium, 2003.
Application of the C18-Carboxypropylbetaine Specimen Processing Method to Recovery of Mycobacterium avium subsp. paratuberculosis from Ruminant Tissue Specimens,Thornton, C.G., et al, J. Clin. Microbiology, May 2002, p. 1783-1790, Vol.40, No.5.
Applied Biosystems User Bulletin. Subject: Sequencing Large DNA Templates.
Klein, R.K., et al, High Throughput BAC DNA Isolation for Physical Map Construction of Sorghum, Plant Molecular Biology Reporter, Kluwer Academic Publishers, 1998.
Concentration And Purification Of Beef Extract Mock Eluates From Water Samples For The Detection Of Enteroviruses Schwab, K.J., et al, Hepatitis A Virus, and Norwalk Virus By Reverse Transcription-PCR, Applied and Env. Microbio., 61:531-537, 1995.
A lymphokine, provisionally designated interleukin T and produced by a human adult T-cell leukemia line, stimulates T-cell proliferation and the induction of lymphokine-activated killer cells PNAS, May 24, 1994 vol. 91 no. 11 4935-4939
Genome-wide sequence and functional analysis of early replicating DNA in normal human fibroblasts Stephanie M Cohen, Terrence S Furey, Norman A Doggett, and David G Kaufman BMC Genomics. 2006; 7: 301. Published online 2006 November 29. doi: 10.1186/1471-2164-7-301.
New Dinucleotide and Trinucleotide Microsatellite Marker Resources for Cotton Genome Research
Umesh K. Reddy Molecular Biology and Physiology Volume 5 / 2001 Issue 2.
DNA Extraction Genetic Diversity as an Indicator of Ecosystem Condition and Sustainability EPA
Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus. Quiniou SM, Katagiri T Genet Sel Evol. 2003 Nov-Dec;35(6):673-83.
Bruce, D.C., et al, BAC Library End Sequencing in Support of Whole Genome Assemblies, poster DOE Joint Genome Institute and Center for Human Genome Studies, Los Alamos National Laboratory. 2002
Detection of Mycobacterium avium Subspecies avium in Formalin-Fixed, Paraffin-Embedded Tissues of Captive Exotic Birds Using Polymerase Chain Reaction Zoltan S. Gyimesi, Ilse H. Stalis, Janice M. Miller and Charles O. Thoen Journal of Zoo and Wildlife Medicine Vol. 30, No. 3 (Sep., 1999), pp. 348-353
Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR. Schwab KJ, Appl Environ Microbiol. 1996 Jun;62(6):2086-94.
A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization LA Jaykus, R De Leon and MD Sobsey Appl. Environ. Microbiol., Jun 1996, 2074-2080, Vol 62, No. 6
Concentration and Purification of Beef Extract Mock Eluates from Water Samples for the Detection of Enteroviruses, Hepatitis A Virus, and Norwalk Virus by Reverse Transcription-PCRAppl. Environ. Microbiol., Feb 1995, 531-537, Vol 61, No. 2 American Society for Microbiology
Application of the C18 -Carboxypropylbetaine Specimen Processing Method to Recovery of Mycobacterium avium subsp.Paratuberculosis from Ruminant Tissue Specimens Appl. Environ. Microbiol., Feb 1995, 531-537, Vol 61, No. 2 KJ Schwab, R De Leon
New Dinucleotide and Trinucleotide Microsatellite Marker Resources for Cotton Genome Research Reddy, O.U.K., et al, Journal of Cotton Science, 5:103-113 (2001).
Huang, G.M., et al, A High-Throughput Plasmid DNA Preparation Method, Analytical Biochem, 223:35-48, 1994.
Kelley, J.M., et al, High Throughput Direct End Sequencing of BAC Clones, Nucleic Acids Research, Vol. 27, No. 6: 1539-1546, 1999.
Miller, J.M., et al, Polymerase chain reaction identification of Mycobacterium avium in formalin-fixed, paraffin-embedded animal tissues, J. Vet. Diagn. Invest. 11:436-440 (1999).