NuGel™ products perform optimally using new on-bead digestion for LC-MS
January 9, 2013, MONMOUTH JUNCTION, NJ- Biotech Support Group reports on a recent development for its line of NuGel™ and related products. With greater interest in the proteomics community for better workflows and performance for LC-MS analyses, affinity separations have often been coupled to direct proteolytic digestion of the bound protein content. This is commonly called “on-bead digestion” in the literature. Biotech Support Group has extended the benefits of on-bead digestion to include its low abundance enrichment products that deplete the high abundance proteins by selective voidance – AlbuVoid™ in the case of albumin from serum/plasma, and HemoVoid™ in the case of hemoglobin from red cell lysates and whole blood. For urine proteins, the UPCK™ Urine Protein Enrichment/Concentration Kit concentrates the proteins sufficiently for on-bead digestion. Furthermore, these same on-bead digestion protocols extend to its affinity sorbents such as KinaSorb™ - for kinases, PDEnRich™ for cyclic nucleotide phosphodiesterases, NuGel™ Glycoprotein Enrichment PBA Kit - With Phenyl Boronic Acid Matrix for glycoproteins, and the NuGel™ covalent chemistries for immobilizing affinity ligands.
“These developments support our efforts to supply ideal enrichment and affinity products for LC-MS proteomics analysis. Our NuGel™ products are different from other protein separations products which are mostly derived from carbohydrate architectures. By contrast, the NuGel™ products are derived from a unique polymer-coated porous silica, which is rigid with a low water content, ideal for concentration and proteolytic digestion. Because of its passivation, the non-specific protein binding is virtually eliminated” states Swapan Roy, Ph.D., President of Biotech Support Group.
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About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of proteomics and genomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Its principal products include: AlbuSorb™ for albumin depletion, AlbuVoid™ for albumin depletion plus low abundance serum protein enrichment, Cleanascite™ for lipid adsorption and clarification, NuGel™ for passivated silica-based affinity chromatography, ProCipitate™ & ProPrep™ for nucleic acid isolation, and for depletion and purifying hemoglobin; HemoVoid™ for depletion from red blood cells and HemogloBind™ primarily for hemolyzed serum. Biotech Support Group LLC and ProFACT Proteomics Inc., are collaborating on the development of a functional proteomics separations platform used in biomarker and functional proteomic prospecting. For more information, go to: http://www.biotechsupportgroup.com