New Research For Cleanascite™

New Research Application For Cleanascite™

Li M. Fu (2011). Functional Analysis of Intergenic Regions for Gene Discovery, Computational Biology and Applied Bioinformatics, Heitor Silverio Lopes and Leonardo Magalhães Cruz (Ed.), ISBN: 978-953-307-629-4, InTech.
Available from: http://www.intechopen.com/articles/show/title/functional-analysis-of-int...

Scientists developed a method for gene finding in intergenic regions of annotated genome which integrated transcription analysis, sequence alignment and homology. Microarray based transcriptional analysis for functional gene finding allowed researchers to discover genes with specific functions such as DNA-binding proteins in CopG family and nickel binding GTPase and figure out functional domains of proteins in active intergenic elements with significant transcription activity.

Scientists from the Pacific Tuberculosis and Cancer Research Organization have cited Cleanascite for dilipidation during RNA isolation in “Functional Analysis of Intergenic Regions for Gene Discovery”. Samples of M. tuberculosis strain H37Rv underwent bacterial lysis and
RNA isolation was performed by mixing cultures in equal volumes of RNA,bacteria collected by centrifugation (1 min, 25000g, 8°C) and the mycobacteria were mechanically disrupted. Cleanascite from Biotech Support Group was used for delipidation of aqueous phase lipids followed by extraction using chloroform-isoamyl alcohol, ethanol precipitation.

For more information about Cleanascite™

Leaves glycoproteins, antibodies, nucleic acids, hemoglobin, proteoglycans, nucleic acids, serum components(such as hormones, nutrients, globulins, clotting factors, transport proteins) alone
Extends the life of membrane and chromatographic columns.
Enrichment of delipidated tissue samples
Ideal for delipidation treatments for downstream processing of large-scale therapeutic proteins, enzymes and monclonal antibodies.

http://biotechsupportgroup.com/cleanascite-lipid-removal-and-clarification