HemoVoid™ - Hemoglobin Depletion From Erythrocytes
HemoVoid™ - Hemoglobin Depletion Reagent Kit
- Hemoglobin voids in flow-through >98%, with <30 minute bind/wash/elute protocol
- Hemoglobin removal from red cell lysates for RBC proteomics
- Hemoglobin removal from hemolyzed serum
- Low abundance protein and enzyme enrichment
- Disposable, cost-effective
- Mild elution maintains tertiary structure and simple transfer to secondary analysis
- Removes hemoglobin from species including human, sheep, bovine, goat, etc.
- The eluted fractions retain their enzymatic and biological activity
HemoVoid™ , a silica-based protein enrichment matrix, removes hemoglobin from erythrocyte lysate samples while concentrating low abundance, and/or low molecular weight proteins. The HemoVoid™ protocol uses mild buffers; the protocol conditions are so gentle that native enzyme activity is retained in elution fractions.
HemoVoid™ derives from a silica-based library of individual mixed-mode ligand combinations (ionic, hydrophobic, aromatic, polymer). The library was designed to facilitate weak binding of proteins, allowing for rapid elution from the matrix without any foreknowledge of the variety of proteins contained in the starting sample. HemoVoid™ depletes hemoglobin from red cell lysates while improving the resolution of less abundant blood proteins.
Click here to view HemoVoid™ Product Sheet
Published Hemovoid™ References
Mizukawa, B., George, A., Pushkaran, S., Weckbach, L., Kalinyak, K., Heubi, J. E. and Kalfa, T. A. , Cooperating G6PD mutations associated with severe neonatal hyperbilirubinemia and cholestasis. Pediatric Blood & Cancer.
Functional 20S proteasomes in mature human red blood cells Sudha Neelam1, David G Kakhniashvili1, Stephan Wilkens1,Stephen D Levene2 and Steven R Goodman1 Exp. Biol. Med. 2011;236:580-591 doi:10.1258/ebm.2011.010394