AlbuTrial Kit™ - AlbuVoid™ and AlbuSorb™

AlbuTrial kit™ includes 1 gm of Albusorb and 5 Preps of AlbuVoid with respective buffers.Matrix facilitating effective removal of albumin from serum. Proteomic analysis of serum and the quest for identifying serum proteins as disease markers have often been hampered by the predominance of proteins like albumin. The unusually high abundance of albumin in serum represses the signals for low abundance proteins, thereby interfering with the resolution and sensitivity of many protein profiling techniques. In the course of evaluating several available methods and commercial kits, we have been able to refine the albumin depletion protocols and establish a modified albumin removal method using ligand based protein fractionation system. The matrices ALBUSORB™ and ALBUVOID™ were tested on human, sheep, bovine, mouse, goat, rat, and calf from serum and plasma using a simple protocol. The procedure is pretty simple, just add, centrifuge and/or filter, and recover the albumin depleted serum. Albusorb™, facilities binding of albumin (>90%) thereby removing rest of the proteins in the flow-through. Albuvoid™, on the other hand flushes majority of albumin (>90%) in the flow-through and wash fractions. In the final elution step the remaining low abundance proteins can be recovered.

Click here for the AlbuTrial kit™ Product Sheet